蛋黄免疫球蛋白 Y 作为一种有前途的工具，可用于检测神经囊尾蚴病血清样本中的免疫复合物。
Egg yolk immunoglobulin Y as a promising tool to detect immune complexes in neurocysticercosis serum samples.
机构信息
Laboratório de Diagnóstico de Parasitoses, Instituto de Ciências Biomédicas, Universidade Federal de Uberlândia, Av. Pará, 1720, Uberlândia, Minas Gerais, 38400-902, Brazil.
Laboratório de Bioquímica e Toxinas Animais, Instituto de Genética e Bioquímica, Universidade Federal de Uberlândia, Av. Pará, 1720, Uberlândia, Minas Gerais, 38400-902, Brazil.
出版信息
Trans R Soc Trop Med Hyg. 2020 Aug 1;114(8):585-592. doi: 10.1093/trstmh/traa028.
BACKGROUND
Neurocysticercosis (NCC) is a neglected tropical disease and its diagnosis is still a challenge due to non-specific manifestations. Neuroimaging techniques are used in the diagnosis of NCC, however, due to the high cost of these methods and the advantages presented in the use of immunological tests, such as ease of performance and satisfactory results, immunoassays are commonly used to detect antibodies against Taenia sp. antigens. The aim of the present study was to produce, characterize and apply specific polyclonal immunoglobulin Y (IgY) anti-Taenia crassiceps extracted from egg yolk of hens immunized with T. crassiceps metacestodes.
METHODS
Indirect enzyme-linked immunosorbent assay (ELISA), avidity ELISA, immunoblotting and indirect immunofluorescence tests were performed for characterization of IgY antibodies. Diagnostic performance was verified by ELISA for immune complex detection testing 90 serum samples.
RESULTS
Values of sensitivity, specificity, positive and negative likelihood ratios (LR+/LR-) and area under the curve (AUC) were calculated and presented the following results: sensitivity 83.3%, specificity 96.7%, AUC 0.966, LR+ 25.0 and LR- 0.17.
CONCLUSIONS
Results of this pioneering and innovative study demonstrate that anti-T. crassiceps IgY antibodies present potential applicability and can be used as an efficient tool in human NCC serodiagnosis.
背景
神经囊尾蚴病（NCC）是一种被忽视的热带病，由于其表现非特异性，因此诊断仍然具有挑战性。神经影像学技术用于诊断 NCC，但是，由于这些方法成本高，并且免疫测试具有易于操作和结果令人满意等优势，因此免疫测定通常用于检测针对带绦虫属抗原的抗体。本研究的目的是从用囊尾蚴感染的母鸡卵黄中提取特异性多克隆免疫球蛋白 Y（IgY）抗猪带绦虫，并对其进行特性分析和应用。
方法
通过间接酶联免疫吸附试验（ELISA）、亲和 ELISA、免疫印迹和间接免疫荧光试验对 IgY 抗体进行特性分析。通过 ELISA 对 90 份血清样本进行免疫复合物检测试验，以验证诊断性能。
结果
计算了敏感性、特异性、阳性和阴性似然比（LR+/LR-）以及曲线下面积（AUC）的值，并得出以下结果：敏感性 83.3%，特异性 96.7%，AUC 为 0.966，LR+为 25.0，LR-为 0.17。
结论
这项开创性和创新性研究的结果表明，抗猪带绦虫 IgY 抗体具有潜在的适用性，可作为人类 NCC 血清学诊断的有效工具。
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