Department of Marine Bio-Materials and Aquaculture, College of Fisheries Sciences, Pukyong National University, 45 Yongso-ro, Nam-gu, Busan 48513, Korea.
Bioinformatics Team, DNA Link Inc., 150 Bugahyeon-ro, Seodaemun-gu, Seoul 03759, Korea.
Int J Mol Sci. 2020 May 29;21(11):3872. doi: 10.3390/ijms21113872.
Maternal genes are important in directing early development and determining egg quality in fish. We here report the de novo transcriptome from four tissue libraries of the cyprinid loach, , and for the first time identified maternal gene transcripts in unfertilized eggs and suggest their immune system involvement. Expression profiles and functional enrichment revealed a total 24,116 transcripts were expressed as maternal transcripts in unfertilized eggs, which were involved in a wide range of biological functions and pathways. Comparison expression profiles and analysis of tissue specificity revealed that the large numbers of maternal transcripts were stored in unfertilized eggs near the late phase of ovarian maturation and before ovulation. Functional classification showed a total of 279 maternal immune-related transcripts classified with immune system process GO term and immune system KEGG pathway. qPCR analysis showed that transcript levels of identified maternal immune-related candidate genes were dynamically modulated during development and early ontogeny of Taken together, this study could not only provide knowledge on the protective roles of maternal immune-related genes during early life stage of but could also be a valuable transcriptomic/genomic resource for further analysis of maternally provisioned genes in and other related teleost fishes.
母体基因在指导鱼类早期发育和决定卵质量方面起着重要作用。我们在这里报告了来自四种组织文库的从头转录组，这是首次在未受精卵中鉴定出母体基因转录本，并提出了它们参与免疫系统的假说。表达谱和功能富集分析显示，共有 24116 个转录本作为母体转录本在未受精卵中表达，这些转录本参与了广泛的生物学功能和途径。比较表达谱和组织特异性分析表明，大量的母体转录本储存在卵巢成熟后期和排卵前的未受精卵中。功能分类显示，总共 279 个母体免疫相关转录本被归类为免疫系统过程 GO 术语和免疫系统 KEGG 途径。qPCR 分析显示，在 的发育和早期胚胎发生过程中，鉴定出的母体免疫相关候选基因的转录水平是动态调节的。综上所述，本研究不仅可以为母体免疫相关基因在 的早期生命阶段的保护作用提供知识，还可以为进一步分析 和其他相关硬骨鱼类中母体提供的基因提供有价值的转录组/基因组资源。
