银屑病患者的皮肤微生物组在接受 TNF 和 IL-17 抑制剂治疗期间得以保留。
Skin Mycobiome of Psoriasis Patients is Retained during Treatment with TNF and IL-17 Inhibitors.
机构信息
Department of Dermatology, Nagasaki University Graduate School of Biomedical Sciences, Nagasaki 852-8523, Japan.
Department of Dermatology, Nagasaki Ekiasaikai Hospital, Nagasaki 850-0034, Japan.
出版信息
Int J Mol Sci. 2020 May 29;21(11):3892. doi: 10.3390/ijms21113892.
BACKGROUND
Biological treatment relieves refractory skin lesions in patients with psoriasis; however, changes in the fungal microbiome (the mycobiome) on the skin are unclear.
METHODS
The skin mycobiome of psoriasis patients treated with TNF inhibitors (TNFi, = 5) and IL-17 inhibitors (IL-17i, = 7) was compared with that of patients not receiving systemic therapy ( = 7). Skin swab samples were collected from non-lesional post-auricular areas. Fungal DNA was sequenced by ITS1 metagenomic analysis and taxonomic classification was performed.
RESULTS
An average of 37543 reads/sample were analyzed and fungi belonging to 31 genera were detected. The genus accounted for >90% of reads in 7/7 samples from the no-therapy group, 4/5 from the TNFi group, and 5/7 from the IL-17i group. Biodiversity was low in those three groups. Few members of the genus were detected; the genus was not detected at all. Among the species, was the major species in 6/7 samples from the no-therapy group, 4/5 from the TNFi group, and 5/7 from the IL-17i group whose the other largest species revealed .
CONCLUSIONS
The mycobiome is retained on post-auricular skin during systemic treatment with TNF and IL-17 inhibitors.
背景
生物治疗可缓解银屑病患者的难治性皮损；然而，皮肤真菌微生物组（真菌组）的变化尚不清楚。
方法
比较了接受 TNF 抑制剂（TNFi，n = 5）和 IL-17 抑制剂（IL-17i，n = 7）治疗的银屑病患者与未接受全身治疗的患者（n = 7）的皮肤真菌组。从非皮损耳后区域采集皮肤拭子样本。通过 ITS1 宏基因组分析对真菌 DNA 进行测序，并进行分类学分类。
结果
平均每个样本分析了 37543 个reads，检测到 31 个属的真菌。属 在无治疗组的 7/7 样本、TNFi 组的 4/5 样本和 IL-17i 组的 5/7 样本中占reads 的>90%。这三组的生物多样性较低。很少检测到属的成员；属根本没有被检测到。在种中，种是无治疗组 6/7 样本、TNFi 组 4/5 样本和 IL-17i 组 5/7 样本中的主要种，其最大的其他种是 。
结论
在使用 TNF 和 IL-17 抑制剂进行全身治疗期间，真菌组保留在后耳皮肤。
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