Key Laboratory of Adaptation and Evolution of Plateau Biota (AEPB), Northwest Institute of Plateau Biology, Chinese Academy of Sciences, Xining 810008, China.
University of Chinese Academy of Sciences, Beijing 100049, China.
Int J Mol Sci. 2020 May 31;21(11):3960. doi: 10.3390/ijms21113960.
Thousand-grain weight (TGW) is a very important yield trait of crops. In the present study, we performed quantitative trait locus (QTL) analysis of TGW in a doubled haploid population obtained from a cross between the bread wheat cultivar "Superb" and the breeding line "M321" using the wheat 55-k single-nucleotide polymorphism (SNP) genotyping assay. A genetic map containing 15,001 SNP markers spanning 2209.64 cM was constructed, and 9 QTLs were mapped to chromosomes 1A, 2D, 4B, 4D, 5A, 5D, 6A, and 6D based on analyses conducted in six experimental environments during 2015-2017. The effects of the QTLs and were shown to be strong and stable in different environments, explaining 15.31-32.43% and 21.34-29.46% of the observed phenotypic variance, and they were mapped within genetic distances of 2.609 cM and 5.256 cM, respectively. These novel QTLs may be used in marker-assisted selection in wheat high-yield breeding.
千粒重（TGW）是作物的一个非常重要的产量性状。在本研究中，我们利用小麦 55-k 单核苷酸多态性（SNP）基因分型检测，对来自面包小麦品种“Superb”和育种系“M321”杂交的双单倍体群体进行了 TGW 的数量性状位点（QTL）分析。构建了一个包含 15001 个 SNP 标记的遗传图谱，覆盖 2209.64cM，并在 2015-2017 年的六个实验环境中进行分析，将 9 个 QTL 定位到染色体 1A、2D、4B、4D、5A、5D、6A 和 6D 上。在不同的环境中，QTL 和 的效应表现出很强且稳定，解释了观测表型方差的 15.31-32.43%和 21.34-29.46%，它们分别位于 2.609cM 和 5.256cM 的遗传距离内。这些新的 QTL 可能在小麦高产育种的标记辅助选择中得到应用。
