Li Shunda, Wang Liang, Meng Yaning, Hao Yuanfeng, Xu Hongxin, Hao Min, Lan Suque, Zhang Yingjun, Lv Liangjie, Zhang Kai, Peng Xiaohui, Lan Caixia, Li Xingpu, Zhang Yelun
College of Plant Science & Technology, Huazhong Agricultural University, No. 1, Shizishan street, Hongshan district, Wuhan 430070, China.
Institute of Cash Crops, Hebei Academy of Agriculture and Forestry Sciences, Shijiazhuang 050051, China.
Plants (Basel). 2021 Apr 7;10(4):713. doi: 10.3390/plants10040713.
Genetic dissection kernel weight-related traits is of great significance for improving wheat yield potential. As one of the three major yield components of wheat, thousand kernel weight (TKW) was mainly affected by grain length (GL) and grain width (GW). To uncover the key loci for these traits, we carried out a quantitative trait loci (QTL) analysis of an F recombinant inbred lines (RILs) population derived from a cross of Henong 5290 (small grain) and 06Dn23 (big grain) with a 50 K single nucleotide polymorphism (SNP) array. A total of 17 stable and big effect QTL, including 5 for TKW, 8 for GL and 4 for GW, were detected on the chromosomes 1B, 2A, 2B, 2D, 4B, 5A, 6A and 6D, respectively. Among these, there were two co-located loci for three traits that were mapped on the chromosome 4BS and 6AL. The QTL on 6AL was the most stable locus and explained 15.4-24.8%, 4.1-8.8% and 15.7-24.4% of TKW, GW and GL variance, respectively. In addition, two more major QTL of GL were located on chromosome arm 2BL and 2DL, accounting for 9.7-17.8% and 13.6-19.8% of phenotypic variance, respectively. In this study, we found one novel co-located QTL associated with GL and TKW in 2DL, /, which could explain 13.6-19.8% and 9.8-10.7% phenotypic variance, respectively. Genetic regions and linked markers of these stable QTL will help to further refine mapping of the corresponding loci and marker-assisted selection (MAS) breeding for wheat grain yield potential improvement.
解析与千粒重相关的性状对提高小麦产量潜力具有重要意义。作为小麦三大产量构成要素之一，千粒重（TKW）主要受粒长（GL）和粒宽（GW）影响。为揭示这些性状的关键基因座，我们利用50K单核苷酸多态性（SNP）芯片对由河农5290（小粒）与06Dn23（大粒）杂交构建的F重组自交系（RILs）群体进行了数量性状基因座（QTL）分析。分别在1B、2A、2B、2D、4B、5A、6A和6D染色体上检测到17个稳定且效应较大的QTL位点，其中5个与TKW相关，8个与GL相关，4个与GW相关。其中，有两个共定位位点涉及三个性状，分别位于4BS和6AL染色体上。位于6AL上的QTL是最稳定的位点，分别解释了TKW、GW和GL变异的15.4 - 24.8%、4.1 - 8.8%和15.7 - 24.4%。此外，另外两个GL的主效QTL分别位于2BL和2DL染色体臂上，分别解释了表型变异的9.7 - 17.8%和13.6 - 19.8%。在本研究中，我们在2DL发现了一个与GL和TKW相关的新的共定位QTL，分别解释了13.6 - 19.8%和9.8 - 10.7%的表型变异。这些稳定QTL的遗传区域和连锁标记将有助于进一步精细定位相应位点，并用于小麦产量潜力改良的分子标记辅助选择（MAS）育种。
