同质 DNA 密码锁可实现多输入一键式检测。

Homogeneous DNA-only keypad locks enable one-pot assay of multi-inputs.

机构信息

Hefei National Laboratory for Physical Sciences at the Microscale, CAS Key Laboratory of Soft Matter Chemistry, iChEM (Collaborative Innovation Center of Chemistry for Energy Materials), Department of Polymer Science and Engineering, University of Science and Technology of China, Hefei, Anhui 230026, P. R. China.

出版信息

Chem Commun (Camb). 2020 Jul 11;56(54):7427-7430. doi: 10.1039/d0cc02868a. Epub 2020 Jun 3.

DOI:10.1039/d0cc02868a

PMID:32490866

Abstract

Homogeneous DNA-only keypad locks were built with multi-stranded scalable junction substrates and a series of double-stranded eliminators to differentially process correctly- and wrongly-added DNA inputs, respectively. Unlike conventional strategies that employed solid-phase platforms, one-pot assay of multiple DNA inputs was achieved, showing merits in fabricating complicated information security systems.

摘要

同源 DNA 键盘锁由多股可扩展结基底和一系列双链消除物构建而成，可分别对正确和错误添加的 DNA 输入进行差分处理。与传统采用固相平台的策略不同，实现了多种 DNA 输入的一锅法检测，在构建复杂信息安全系统方面具有优势。