Nascimento Lízia Daniela E Silva, Nicolau Renata Amadei, Maia Filho Antônio Luiz Martins, Nascimento Kárita Francisca E Silva, Santos José Zilton Lima Verde, Sousa Rayssilane Cardoso de, Carvalho Luiz Fernando Meneses, Viana Vicente Galber Freitas
Health Sciences Center, Universidade do Vale do Paraíba, Teresina, PI, Brazil.
Postgraduate Program in Biomedical Engineering of the Research and Development Institute, Research and Development Institute, UNIVAP, Sao Jose dos Campos, SP, Brazil.
Acta Cir Bras. 2020;35(3):e202000303. doi: 10.1590/s0102-865020200030000003. Epub 2020 May 22.
To evaluate the in vivo response of photobiomodulation therapy associated with norbixin-based poly(hydroxybutyrate) membrane (PHB) in tenotomized calcaneal tendon.
Thirty rats were randomly allocated to six groups (n=5 each): LED groups (L1, L2 and L3) and membrane + LED groups (ML1, ML2 and ML3). The right calcaneal tendons of all animals were sectioned transversely and were irradiated with LED daily, one hour after surgery every 24 hours, until the day of euthanasia. At the end of the experiments the tendons were removed for histological analysis.
The histological analysis showed a significant reduction in inflammatory cells in the ML1, ML2 and ML3 groups (p=0.0056, p=0.0018 and p<0.0001, respectively) compared to those in the LED group. There was greater proliferation of fibroblasts in the ML1 (p<0.0001) and L3 (p<0.0001) groups. A higher concentration of type I collagen was also observed in the ML1 group (p=0.0043) replacing type III collagen.
Photobiomodulation in association with norbixin-based PHB membrane led to control of the inflammatory process. However, it did not favor fibroblast proliferation and did not optimize type I collagen formation in the expected stage of the repair process.
评估基于降胭脂树素的聚羟基丁酸酯膜（PHB）联合光生物调节疗法在跟腱切断术后的体内反应。
将30只大鼠随机分为六组（每组n = 5）：LED组（L1、L2和L3）和膜+ LED组（ML1、ML2和ML3）。所有动物的右跟腱均横向切断，并在术后每24小时于术后1小时用LED照射，直至安乐死当天。实验结束时，取出肌腱进行组织学分析。
组织学分析显示，与LED组相比，ML1、ML2和ML3组中的炎性细胞显著减少（分别为p = 0.0056、p = 0.0018和p < 0.0001）。ML1组（p < 0.0001）和L3组（p < 0.0001）中成纤维细胞的增殖更多。在ML1组中还观察到I型胶原蛋白的浓度更高（p = 0.0043），取代了III型胶原蛋白。
基于降胭脂树素的PHB膜联合光生物调节疗法可控制炎症过程。然而，它不利于成纤维细胞增殖，并且在修复过程的预期阶段未优化I型胶原蛋白的形成。
