Department of Physical Therapy, Federal University of Jequitinhonha and Mucuri Valleys, UFVJM, Campus JK - MG Rodovia MGT 367 - Km 583, n° 5000 - Alto da Jacuba, Diamantina, 39100-000, Brazil.
School of Medicine, Federal University of Jequitinhonha and Mucuri Valleys, UFVJM, Campus JK, Diamantina, MG, Brazil.
Lasers Med Sci. 2021 Aug;36(6):1201-1208. doi: 10.1007/s10103-020-03155-3. Epub 2020 Oct 10.
The aim of the present study was to investigate the effects of photobiomodulation (PBM) therapy on the expression of heat shock protein 70 (HSP70) and tissue repair in an experimental model of collagenase-induced Achilles tendinitis. Thirty Wistar rats (aged 12 weeks) were randomly distributed among control group (n = 8), tendinitis group (n = 11), and LED group (n = 11). Tendinitis was induced in the tendinitis and LED groups through a peritendinous injection of collagenase (100 μl). The LED group animals received the first irradiation 1 h after injury. A 630 ± 20 nm, 300-mW continuous wave light-emitting diode (LED), spot size 1 cm, was placed in contact with the skin. One point over the tendon was irradiated for 30 s, delivering 9 J (9 J/cm). LED irradiation was performed once daily for 7 days, with the total energy delivered being 63 J. The tendons were surgically removed and expression of the HSP70 protein was calculated using semi-quantitative analyses of immunohistochemistry (HSCORE). Number of fibroblasts and amount of collagen were measured using histological and histochemical analyses. An increase in the mean HSCORE for HSP70, in the number of fibroblasts, and in the amount of collagen were found in the LED group compared with those in the tendinitis and control group (P ≤ 0.05). PBM therapy increased the expression of the HSP70, number of fibroblasts, and amount of collagen in the acute Achilles tendinitis in rats.
本研究旨在探讨光生物调节（PBM）疗法对胶原酶诱导的跟腱炎实验模型中热休克蛋白 70（HSP70）表达和组织修复的影响。30 只 Wistar 大鼠（12 周龄）随机分为对照组（n=8）、跟腱炎组（n=11）和 LED 组（n=11）。在跟腱炎和 LED 组中，通过腱周注射胶原酶（100 μl）诱导跟腱炎。LED 组动物在损伤后 1 小时接受第一次照射。使用波长为 630±20nm、功率为 300mW 的连续波发光二极管（LED），光斑大小为 1cm，与皮肤接触。在肌腱上照射 1 点，照射 30s，输送 9J（9J/cm）。每天进行 1 次 LED 照射，共 7 天，总能量为 63J。通过免疫组织化学（HSCORE）半定量分析计算 HSP70 蛋白的表达，通过组织学和组织化学分析测量成纤维细胞数量和胶原含量。与跟腱炎组和对照组相比，LED 组 HSP70 的平均 HSCORE、成纤维细胞数量和胶原含量增加（P≤0.05）。PBM 治疗可增加急性跟腱炎大鼠 HSP70、成纤维细胞数量和胶原含量的表达。
