Department of Horticulture and Landscape Architecture, Center for Rhizosphere Biology, Colorado State University, Fort Collins, Colorado, United States of America.
Jiangsu Provincial Key Lab of Organic Solid Waste Utilization, Jiangsu Collaborative Innovation Center for Organic Solid Waste Utilization, Nanjing Agricultural University, Nanjing, China.
PLoS One. 2020 Jun 3;15(6):e0234216. doi: 10.1371/journal.pone.0234216. eCollection 2020.
The role of root exudates has long been recognized for its potential to improve nutrient use efficiency in cropping systems. However, studies addressing the variability of root exudates involved in phosphorus solubilization across plant developmental stages remain scarce. Here, we grew Arabidopsis thaliana seedlings in sterile liquid culture with a low, medium, or high concentration of phosphate and measured the composition of the root exudate at seedling, vegetative, and bolting stages. The exudates changed in response to the incremental addition of phosphorus, starting from the vegetative stage. Specific metabolites decreased in relation to phosphate concentration supplementation at specific stages of development. Some of those metabolites were tested for their phosphate solubilizing activity, and 3-hydroxypropionic acid, malic acid, and nicotinic acid were able to solubilize calcium phosphate from both solid and liquid media. In summary, our data suggest that plants can release distinct compounds to deal with phosphorus deficiency needs influenced by the phosphorus nutritional status at varying developmental stages.
根分泌物在提高作物系统养分利用效率方面的作用早已得到认可。然而，关于植物发育阶段不同磷溶解相关根分泌物变异性的研究仍然很少。在这里，我们在含有低、中、高浓度磷酸盐的无菌液体培养基中培养拟南芥幼苗，并在幼苗、营养生长和抽薹阶段测量根分泌物的组成。根分泌物随着磷的逐步添加而发生变化，从营养生长阶段开始。在特定的发育阶段，与磷酸盐浓度补充相关的特定代谢物减少。对其中一些代谢物进行了磷酸盐溶解活性测试，发现 3-羟基丙酸、苹果酸和烟酸能够从固体和液体培养基中溶解磷酸钙。总之，我们的数据表明，植物可以释放出不同的化合物来应对不同发育阶段磷营养状况影响的磷缺乏需求。
