Umar Mohammad Faisal, Ahmad Faizan, Saeed Haris, Usmani Saad Ali, Owais Mohammad, Rafatullah Mohd
School of Industrial Technology, Universiti Sains Malaysia, Penang 11800, Malaysia.
Department of Post Harvest Engineering and Technology, Faculty of Agricultural Sciences, Aligarh Muslim University, Aligarh 202002, India.
Nanomaterials (Basel). 2020 Jun 1;10(6):1096. doi: 10.3390/nano10061096.
A novel method of preparing reduced graphene oxide (RGOX) from graphene oxide (GOX) was developed employing vegetable extract, , as a reducing and stabilizing agent. is a green leafy vegetable with a low shelf life, fresh leaves of this vegetable are encouraged to be used due to high water content. The previously modified 'Hummers method' has been in practice for the preparation of GOX by using precursor graphite powder. In this study, green synthesis of RGOX was functionally verified by employing FTIR and UV-visible spectroscopy, along with SEM and TEM. Our results demonstrated typical morphology of RGOX stacked in layers that appeared as silky, transparent, and rippled. The antibacterial activity was shown by analyzing minimal inhibitory concentration values, agar diffusion assay, fluorescence techniques. It showed enhanced antibacterial activity against Gram-positive and Gram-negative bacteria in comparison to GOX. It has also been shown that the synthesized compound exhibited enhanced antibiofilm activity as compared to its parent compound. The efficacy of RGOX and GOX has been demonstrated on a human breast cancer cell line, which suggested RGOX as a potential anticancer agent.
开发了一种以植物提取物作为还原和稳定剂，从氧化石墨烯（GOX）制备还原氧化石墨烯（RGOX）的新方法。 是一种保质期短的绿叶蔬菜，由于含水量高，鼓励使用这种蔬菜的新鲜叶子。先前改进的“哈默斯法”已用于通过使用前驱体石墨粉制备GOX。在本研究中，通过傅里叶变换红外光谱（FTIR）和紫外可见光谱，以及扫描电子显微镜（SEM）和透射电子显微镜（TEM）对RGOX的绿色合成进行了功能验证。我们的结果表明，RGOX的典型形态是层状堆积，呈现出丝滑、透明和波纹状。通过分析最低抑菌浓度值、琼脂扩散试验、荧光技术显示了抗菌活性。与GOX相比，它对革兰氏阳性菌和革兰氏阴性菌表现出增强的抗菌活性。还表明，合成的化合物与其母体化合物相比表现出增强的抗生物膜活性。RGOX和GOX对人乳腺癌细胞系的疗效已得到证实，这表明RGOX是一种潜在的抗癌剂。
