Mujeeb Anzar Abdul, Khan Nuha Abeer, Jamal Fauzia, Badre Alam Khan Farheen, Saeed Haris, Kazmi Shadab, Alshameri Ansam Wadia Faid, Kashif Mohammad, Ghazi Irfan, Owais Mohammad
Interdisciplinary Biotechnology Unit, Aligarh Muslim University, Aligarh, India.
Plant Molecular Biology and Genetic Engineering Division, The National Botanical Research Institute, Council of Scientific and Industrial Research, Lucknow, India.
Front Chem. 2020 Feb 28;8:103. doi: 10.3389/fchem.2020.00103. eCollection 2020.
In the present study, we have synthesized silver-copper nanocomposites (Ag-Cu NCs) using an leaf extract (green synthesis method) and evaluated their antimicrobial potential against less susceptible pathogens. The kinetics of Ag-Cu NCs synthesis was followed by UV-VIS and fluorescence spectroscopy. The physicochemical characterization of as-synthesized Ag-Cu NCs was executed using electron microscopy, Energy Dispersive X-Ray, Fourier Transform Infrared Spectroscopy, and a Differential Light Scattering method. As-synthesized Ag-Cu NCs induced the formation of Reactive Oxygen Species (ROS), thereby causing alteration and decrementation of cellular proteins, DNA, lipids, etc., and eventually leading to cell death, as determined by a Live/Dead assay. Next, we assessed the anti-biofilm potential of as-synthesized Ag-Cu NCs against biofilm forming bacteria. The as-synthesized Ag-Cu NCs, when compared to monometallic silver nanoparticles, exhibited significantly higher anti-microbial activity against both sensitive as well as drug resistant microbial isolates.
在本研究中，我们使用叶提取物（绿色合成法）合成了银铜纳米复合材料（Ag-Cu NCs），并评估了它们对较难感染病原体的抗菌潜力。通过紫外可见光谱和荧光光谱跟踪Ag-Cu NCs的合成动力学。使用电子显微镜、能量色散X射线、傅里叶变换红外光谱和差示光散射法对合成后的Ag-Cu NCs进行物理化学表征。如活/死分析所确定的，合成后的Ag-Cu NCs诱导活性氧（ROS）的形成，从而导致细胞蛋白质、DNA、脂质等发生改变和减少，最终导致细胞死亡。接下来，我们评估了合成后的Ag-Cu NCs对形成生物膜细菌的抗生物膜潜力。与单金属银纳米颗粒相比，合成后的Ag-Cu NCs对敏感和耐药微生物分离株均表现出显著更高的抗菌活性。
