Department of Pharmacy, Shantou University Medical College, No. 22 Xinling Road, Shantou, 515041, China.
Department of Pharmacy, Shantou University Medical College, No. 22 Xinling Road, Shantou, 515041, China.
Anal Chim Acta. 2020 Jul 18;1121:1-10. doi: 10.1016/j.aca.2020.04.072. Epub 2020 Apr 30.
Many polymer decorated/modified 2D nanomaterials have been developed as enhanced drug delivery systems and photothermal theranostic nanoagents. However, few reports describe the use of these novel nanomaterials as nanoplatforms for biomolecule sensing. Herein, we used calcium-cation-doped polydopamine-modified (PDA-modified) 2D black phosphorus (BP) nanosheets (BP@PDA) as a sensing nanoplatform for the detection of nucleic acids and proteins in complex biological samples. Fluorescent-dye-labeled single-strand DNA aptamer/probes are adsorbed by the Ca-doped BP@PDA mediated by calcium-cation coordination. The PDA coating enhances the stability of the inner BP, provides binding sites to DNA nucleobases, and quenches fluorescence. Without any chemical conjugation, this sensing nanoplatform selectively and specifically detects protein (human thrombin, linear range: 10-25 nM, detection limit: 0.02 nM), single-strand DNA (linear range: 1-10 nM, detection limit: 0.52 nM) in 1% serum diluted samples, and senses intracellular mRNAs (C-myc, and actin) in living cells. The nanoplatform exhibits the advantages of both the 2D nanomaterial (BP) and the coating polymer (PDA), naturally enters living cells unaided by transfection agents, resists enzymatic lysis and shows high biocompatibility. This nanoplatform design contributes towards future biomolecule analytical method development based on polymer decorated/modified 2D nanomaterials.
许多聚合物修饰/改性的二维纳米材料已被开发为增强型药物传递系统和光热治疗诊断纳米制剂。然而，很少有报道描述这些新型纳米材料作为生物分子传感的纳米平台。在此，我们使用钙掺杂的聚多巴胺修饰（PDA 修饰）二维黑磷（BP）纳米片（BP@PDA）作为传感纳米平台，用于检测复杂生物样品中的核酸和蛋白质。荧光染料标记的单链 DNA 适体/探针通过钙掺杂 BP@PDA 介导的钙阳离子配位被吸附。PDA 涂层增强了内 BP 的稳定性，提供了与 DNA 碱基的结合位点，并猝灭了荧光。在没有任何化学偶联的情况下，这种传感纳米平台选择性和特异性地检测蛋白质（人凝血酶，线性范围：10-25 nM，检测限：0.02 nM）、1%血清稀释样品中的单链 DNA（线性范围：1-10 nM，检测限：0.52 nM），以及在活细胞中检测细胞内 mRNAs（C-myc 和肌动蛋白）。该纳米平台结合了二维纳米材料（BP）和涂层聚合物（PDA）的优点，无需转染试剂即可自然进入活细胞，抵抗酶裂解并表现出高生物相容性。这种纳米平台的设计为基于聚合物修饰的二维纳米材料的未来生物分子分析方法的发展做出了贡献。
