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从锦葵科植物中分离得到的sp.菌株UICC B-92中吩嗪衍生物的分离与结构解析

Isolation and structure elucidation of phenazine derivative from sp. strain UICC B-92 isolated from (Malvaceae).

作者信息

Pratiwi Rina Hidayati, Hidayat Iman, Hanafi Muhammad, Mangunwardoyo Wibowo

机构信息

Department of Mathematics and Natural Sciences, Faculty of Post Graduated, Universitas Indraprasta PGRI, South Jakarta, Indonesia.

Department of Microbiology, Research Center for Biology, Indonesian Institute of Sciences (LIPI), Cibinong, Indonesia.

出版信息

Iran J Microbiol. 2020 Apr;12(2):127-137.

PMID:32494347
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7244822/
Abstract

BACKGROUND AND OBJECTIVES

Endophytic actinomycetes have been known as a promising source for new antibiotics discovery against susceptible and resistant forms of pathogenic microorganisms. This study was aimed at determining antibacterial compound from sp. strain B-92 isolated from a medicinal plant .

MATERIALS AND METHODS

sp. strain UICC B-92 was endophytic actinomycetes of that obtained from Universitas Indonesia Culture Collection (UICC). Isolation and determination of bioactive compound were carried out using thin layer chromatography (TLC), nuclear magnetic resonance spectroscopy (NMR), and liquid chromatography mass spectrometry (LC-MS) analyses. An antibacterial assay of pure bioactive compound from the endophytic actinomycetes strain was performed against strain ATCC 10876, strain ATCC 25922, strain ATCC 25241, strain ATCC 12022 and strain ATCC 25923.

RESULTS

The bioactive compound was identified as 4-((3S,4R,5S)-3,4,5-trihydroxy-6-(hydroxymethyl) tetrahydro-2H-pyran-2-yloxy) phenazine-1-carboxylic acid. antimicrobial assay showed that bioactive compound of sp. strain UICC B-92 exhibited antagonistic activities against two Gram-positive bacteria, viz, strain ATCC 10876 and strain ATCC 25923.

CONCLUSION

The findings of this research showed that, bioactive compound of sp. strain UICC B-92 is suggested a new compound based on glycoside structure and its position.

摘要

背景与目的

内生放线菌已被认为是发现针对致病性微生物敏感和耐药形式的新型抗生素的一个有前景的来源。本研究旨在从一种药用植物中分离得到的sp.菌株B-92中确定抗菌化合物。

材料与方法

sp.菌株UICC B-92是从印度尼西亚大学培养物保藏中心（UICC）获得的内生放线菌。使用薄层色谱（TLC）、核磁共振光谱（NMR）和液相色谱-质谱（LC-MS）分析进行生物活性化合物的分离和测定。对来自内生放线菌菌株的纯生物活性化合物进行了针对ATCC 10876菌株、ATCC 25922菌株、ATCC 25241菌株、ATCC 12022菌株和ATCC 25923菌株的抗菌试验。

结果

该生物活性化合物被鉴定为4-((3S,4R,5S)-3,4,5-三羟基-6-(羟甲基)四氢-2H-吡喃-2-基氧基)吩嗪-1-羧酸。抗菌试验表明，sp.菌株UICC B-92的生物活性化合物对两种革兰氏阳性菌，即ATCC 10876菌株和ATCC 25923菌株表现出拮抗活性。

结论

本研究结果表明，sp.菌株UICC B-92的生物活性化合物基于糖苷结构及其位置被认为是一种新化合物。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/71b0/7244822/51741cde3384/IJM-12-127-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/71b0/7244822/4a0b894ccf14/IJM-12-127-g001.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/71b0/7244822/910e66ca57be/IJM-12-127-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/71b0/7244822/c152a3e0ab64/IJM-12-127-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/71b0/7244822/581b6f9d777c/IJM-12-127-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/71b0/7244822/efd0ade28cd0/IJM-12-127-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/71b0/7244822/afb24dc52bc7/IJM-12-127-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/71b0/7244822/51741cde3384/IJM-12-127-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/71b0/7244822/4a0b894ccf14/IJM-12-127-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/71b0/7244822/d9eedf7620a1/IJM-12-127-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/71b0/7244822/910e66ca57be/IJM-12-127-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/71b0/7244822/c152a3e0ab64/IJM-12-127-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/71b0/7244822/581b6f9d777c/IJM-12-127-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/71b0/7244822/efd0ade28cd0/IJM-12-127-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/71b0/7244822/afb24dc52bc7/IJM-12-127-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/71b0/7244822/51741cde3384/IJM-12-127-g008.jpg

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