Department of Thoracic Surgery, First Affiliated Hospital of Fujian Medical University, Fuzhou City, Fujian Province, P.R. China.
Eur Rev Med Pharmacol Sci. 2020 May;24(10):5412-5418. doi: 10.26355/eurrev_202005_21325.
Identification of novel and reliable biomarkers is crucial for the early detection and prognosis prediction of esophageal squamous cell carcinoma (ESCC). In this study, we aimed to explore the potential clinical significance of serum exosomal miR-182 in ESCC.
A total of 125 patients with ESCC and 60 healthy volunteers were enrolled in this study. Quantitative reverse transcription-polymerase chain reaction (qRT-PCR) was used to detect the serum exosomal miR-182 level. Then, the associations between serum exosomal miR-182 levels and clinicopathological features, as well as clinical outcome were further investigated.
Serum exosomal miR-182 levels were significantly higher in pre-operative ESCC patients than in normal controls and post-operative ESCC patients. In addition, the receiver operating characteristic (ROC) curve analysis showed that the serum exosomal miR-182 could well differentiate ESCC patients from the healthy controls. Moreover, high serum exosomal miR-182 expression was strongly associated with worse clinical parameters including differentiation, lymph node metastasis, and TNM stage. ESCC patients in the high serum exosomal miR-182 group had significantly shorter overall survival and relapse free survival than those in the low serum exosomal miR-182 group. Furthermore, serum exosomal miR-182 was an independent prognostic indicator for ESCC.
Collectively, serum exosomal miR-182 might serve as a promising biomarker for predicting the unfavorable prognosis of ESCC.
鉴定新型和可靠的生物标志物对于食管鳞癌（ESCC）的早期检测和预后预测至关重要。在本研究中，我们旨在探讨血清外泌体 miR-182 在 ESCC 中的潜在临床意义。
本研究共纳入 125 例 ESCC 患者和 60 名健康志愿者。采用定量逆转录-聚合酶链反应（qRT-PCR）检测血清外泌体 miR-182 水平。然后，进一步研究血清外泌体 miR-182 水平与临床病理特征及临床结局的关系。
术前 ESCC 患者血清外泌体 miR-182 水平明显高于正常对照组和术后 ESCC 患者。此外，受试者工作特征（ROC）曲线分析表明，血清外泌体 miR-182 可较好地区分 ESCC 患者与健康对照者。此外，高血清外泌体 miR-182 表达与较差的临床参数密切相关，包括分化程度、淋巴结转移和 TNM 分期。高血清外泌体 miR-182 组的 ESCC 患者总生存和无复发生存均明显短于低血清外泌体 miR-182 组。此外，血清外泌体 miR-182 是 ESCC 的独立预后指标。
总之，血清外泌体 miR-182 可能是预测 ESCC 不良预后的有前途的生物标志物。
