Centre SÈVE, Département de biologie, Faculté des sciences, Université de Sherbrooke, 2500 boulevard de l'Université, Sherbrooke, QC J1K 2R1, Canada.
Can J Microbiol. 2020 Nov;66(11):664-669. doi: 10.1139/cjm-2020-0174. Epub 2020 Jun 4.
and are used in organic agriculture as an alternative to chemical pesticides to fight against phytopathogen organisms. These Gram-positive soil-dwelling bacteria are able to resist harsh conditions and survive by differentiating into endospores. Few studies have examined how bacterial populations change on plants over time, and whether they remain active or enter a dormant state. Nonetheless, these characteristics are strikingly important to determine the usage of and and their efficacy in environmental conditions. Here, we investigated the population dynamics of NCIB3610 and QST713 when applied as spores on different ornamental plants. We report that on all the plants studied ( 'Salsa red', 'Fatal attraction', and 'Hidecote blue'), spores rapidly germinated and colonized the rhizoplane, maintaining a relatively low proportion of spores in the population over time, whereas the bacterial population on the leaves rapidly declined. Bacteria in the surrounding soil did not germinate and persisted as spores. Taken together, these results suggest that only cells found at the rhizosphere remain metabolically active to allow the formation of a lasting relationship with the plant, making possible beneficial effects from the inoculated bacteria.
和 被广泛应用于有机农业，作为化学农药的替代品，以对抗植物病原菌。这些革兰氏阳性的土壤栖息细菌能够通过分化为内生孢子来抵抗恶劣的环境条件并存活下来。很少有研究调查过随着时间的推移，植物上的细菌种群如何变化，以及它们是保持活跃状态还是进入休眠状态。尽管如此，这些特性对于确定 和 的使用以及它们在环境条件下的功效是非常重要的。在这里，我们研究了作为孢子施用于不同观赏植物时，NCIB3610 和 QST713 的种群动态。我们报告说，在所研究的所有植物上（“Salsa red”、“Fatal attraction”和“Hidecote blue”），孢子迅速发芽并定殖于根际，随着时间的推移，种群中保持相对较低比例的孢子，而叶片上的细菌种群迅速减少。周围土壤中的细菌没有发芽并作为孢子存活下来。总之，这些结果表明，只有在根际发现的细胞保持代谢活性，才能与植物形成持久的关系，从而使接种细菌产生有益的影响成为可能。
