评估含肠球菌的商业兽医益生菌是否具有可转移的万古霉素耐药基因。
Evaluation of commercial veterinary probiotics containing enterococci for transferrable vancomycin resistance genes.
机构信息
Department of Veterinary Clinical Sciences, College of Veterinary Medicine, Washington State University, Pullman, WA, 99163, USA.
Department of Veterinary Clinical Sciences, Iowa State University, Ames, IA, 50010, USA.
出版信息
BMC Res Notes. 2020 Jun 4;13(1):275. doi: 10.1186/s13104-020-05114-1.
Abstract
OBJECTIVE
Vancomycin resistant enterococci (VRE) are of significant public health concern. The identification of VRE in livestock and food has increased. The objective of this study was to determine if the transferrable vancomycin resistance genes vanA and vanB were present in probiotics marketed for use in animals that claimed to contain Enterococcus spp.
RESULTS
Of the 40 products selected, Enterococcus spp. DNA was successfully extracted from 36 products. Of these 36 products with enterococcal DNA, 2 (6%) had a PCR product consistent with vanA which was confirmed by sequencing. None of the products appeared to contain vanB.
摘要
目的
耐万古霉素肠球菌（VRE）是一个重大的公共卫生问题。在牲畜和食品中发现的耐万古霉素肠球菌数量不断增加。本研究的目的是确定是否在声称含有肠球菌属的动物用益生菌中存在可转移的万古霉素耐药基因 vanA 和 vanB。
结果
在选择的 40 种产品中，成功从 36 种产品中提取了肠球菌属 DNA。在这 36 种含有肠球菌属 DNA 的产品中，有 2 种（6%）的 PCR 产物与 vanA 一致，测序结果得到了证实。没有一种产品似乎含有 vanB。
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