Department of Pharmacy, Inner Mongolia Medical University, Jinshan Development Zone, Hohhot, 010110, China.
Department of Pharmacy, Inner Mongolia Medical University, Jinshan Development Zone, Hohhot, 010110, China.
J Ethnopharmacol. 2020 Oct 5;260:113018. doi: 10.1016/j.jep.2020.113018. Epub 2020 Jun 2.
Echinops latifolius Tausch (ELT) is traditional Mongolian medicine in China, and often used to against osteoporosis, strengthen tendons and bones, clear bones heat.
To study efficacy of ELT on ovariectomized (OVX) rats and underly metabolic pathways related to trabecular micro-architecture changing of OVX.
Three-month-old female Wistar rats were randomly divided into 4 groups (n = 6) including normal group (without surgery), sham group (bilateral laparotomy), OVX group (bilateral ovariectomy), and ELT-treated groups (ELT-treated after bilateral ovariectomy). The effects of ELT on trabecular micro-architecture and biochemical markers of OVX rat were investigated by dual-energy X-ray absorptiometry machine and Enzyme-linked immunosorbent assay (ELISA), respectively. Untargeted metabolomics strategy was applied to discover the potential biomarkers and related metabolic pathways involving the progression of OVX-induced osteoporosis.
The trabecular micro-architecture and biochemical markers of OVX rats were improved by ELT. We found 36 potential biomarkers and 21 related metabolic pathways were involved in progression of OVX-induced osteoporosis. Amino acids metabolism and glycerophospholipids metabolism were mainly intervened in ELT treatment on ovariectomized rats. The disordered amino acids and glycerophospholipids metabolism closely related to the imbalance between bone resorption and formation were reversed by administration of ELT, indicating that the influences of ELT on OVX rats' trabecular micro-architecture may possible be associated with intervening amino acids and glycerophospholipids metabolism.
This approach may provide the metabolomic perspective to link metabolic alterations and anti-osteoporosis action of ELT, to further explain how ELT works in postmenopausal patients with bone loss.
在中国，Echinops latifolius Tausch（ELT）是一种传统的蒙药，常用于治疗骨质疏松症、强筋健骨、清除骨热。
研究 ELT 对去卵巢（OVX）大鼠的疗效及其与去卵巢导致的小梁微观结构变化相关的代谢途径。
将 3 月龄雌性 Wistar 大鼠随机分为 4 组（n=6），包括正常组（未手术）、假手术组（双侧剖腹术）、OVX 组（双侧卵巢切除术）和 ELT 治疗组（双侧卵巢切除术后给予 ELT 治疗）。采用双能 X 射线吸收仪和酶联免疫吸附试验（ELISA）分别研究 ELT 对 OVX 大鼠小梁微观结构和生化标志物的影响。采用非靶向代谢组学策略发现涉及 OVX 诱导骨质疏松症进展的潜在生物标志物和相关代谢途径。
ELT 改善了 OVX 大鼠的小梁微观结构和生化标志物。我们发现了 36 个潜在的生物标志物和 21 个相关的代谢途径参与了 OVX 诱导的骨质疏松症的进展。氨基酸代谢和甘油磷脂代谢是 ELT 治疗去卵巢大鼠的主要干预靶点。ELT 治疗可纠正紊乱的氨基酸和甘油磷脂代谢，这些代谢与骨吸收和形成之间的失衡密切相关，表明 ELT 对 OVX 大鼠小梁微观结构的影响可能与干预氨基酸和甘油磷脂代谢有关。
该方法可能为代谢组学提供一个视角，将代谢变化与 ELT 的抗骨质疏松作用联系起来，进一步解释 ELT 如何在绝经后骨质疏松症患者中发挥作用。
