National Demonstration Center for Experimental Plant Science Education, College of Agriculture, Guangxi University, Nanning 530004, PR China.
Pharmaceutical College, Guangxi Medical University, Nanning 530021, PR China.
Microbiol Res. 2020 Sep;238:126500. doi: 10.1016/j.micres.2020.126500. Epub 2020 May 26.
Ralstonia solanacearum is a scientifically/economically important plant pathogenic bacterium. The plant disease caused by R. solanacearum causes huge economic losses, and efficient control measures for the disease remain limited. To gain a better system-level understanding of R. solanacearum, we generated a near-saturated transposon insertion library of R. solanacearum GMI1000 with approximately 240,000 individual insertion mutants. Transposon sequencing (Tn-seq) allowed the mapping of 70.44%-80.96% of all potential insertion sites of the mariner C9 transposase in the genome of R. solanacearum and the identification of 465 genes essential for the growth of R. solanacearum in rich medium. Functional and comparative analyses of essential genes revealed that many basic physiological and biochemical processes such as transcription differ between R. solanacearum and other bacteria. A comparative analysis of essential genes also suggested that 34 genes might be essential only for Ralstonia group bacteria, whereas another 16 essential genes are unique to Ralstonia, providing high-priority candidate targets for developing R. solanacearum-specific drugs.
青枯雷尔氏菌是一种具有重要科学和经济意义的植物病原细菌。由青枯雷尔氏菌引起的植物病害会造成巨大的经济损失，但目前针对该病害的有效防治措施仍然十分有限。为了更好地从系统水平上理解青枯雷尔氏菌，我们构建了一个近乎饱和的青枯雷尔氏菌 GMI1000 转座子插入突变体库，其中包含约 24 万个单插入突变体。转座子测序（Tn-seq）可以定位 mariner C9 转座酶在青枯雷尔氏菌基因组中的潜在插入位点，我们在青枯雷尔氏菌基因组中鉴定出了 465 个对其在丰富培养基中生长至关重要的基因。对必需基因的功能和比较分析表明，转录等许多基本的生理和生化过程在青枯雷尔氏菌和其他细菌之间存在差异。对必需基因的比较分析还表明，34 个基因可能仅对雷尔氏菌属细菌是必需的，而另外 16 个必需基因是雷尔氏菌属所特有的，这为开发青枯雷尔氏菌特异性药物提供了高优先级的候选靶标。
