Hong Inpyo, Pae Hyung-Chul, Song Young Woo, Cha Jae-Kook, Lee Jung-Seok, Paik Jeong-Won, Choi Seong-Ho
Department of Periodontology, Research Institute of Periodontal Regeneration, Yonsei University College of Dentistry, Seoul 120-749, Korea.
J Clin Med. 2020 Jun 3;9(6):1720. doi: 10.3390/jcm9061720.
Diagnoses based on oral fluid biomarkers have been introduced to overcome limitations of periodontal probe-based diagnoses. Diagnostic ability of certain biomarkers for periodontitis have been identified and widely studied, however, such studies targeting gingivitis is scarce. The aims of this study were to determine and compare the efficacies and accuracies of eight biomarkers in diagnosing gingivitis with the aid of receiver operating characteristic (ROC) curves. The probing depth (PD), clinical attachment loss (CAL), bleeding on probing (BOP), gingival index (GI), and plaque index (PI) were examined in 100 participants. Gingival crevicular fluid was collected using paper points, and whole-saliva samples were collected using cotton roll. Samples were analyzed using enzyme-linked immunosorbent assay kits for the different biomarkers. The levels of matrix metalloproteinase (MMP)-8, MMP-9, lactoferrin, cystatin C, myeloperoxidase (MPO), platelet-activating factor, cathepsin B, and pyridinoline cross-linked carboxyterminal telopeptide of type I collagen were analyzed. MPO and MMP-8 levels in saliva were strongly correlated with gingivitis, with Pearson's correlation coefficients of 0.399 and 0.217, respectively. The area under the curve (AUC) was largest for MMP-8, at 0.814, followed by values of 0.793 and 0.777 for MPO and MMP-9, respectively. The clinical parameters of GI and PI showed strong correlations and large AUC values, whereas PD and CAL did not. MMP-8 and MPO were found to be effective for diagnosing gingivitis. Further investigations based on the results of this study may identify clinically useful biomarkers for the accurate and early detection of gingivitis.
基于口腔液体生物标志物的诊断方法已被引入，以克服基于牙周探针诊断的局限性。某些牙周炎生物标志物的诊断能力已得到确认并被广泛研究，然而，针对牙龈炎的此类研究却很少。本研究的目的是借助受试者工作特征（ROC）曲线来确定和比较八种生物标志物在诊断牙龈炎方面的有效性和准确性。对100名参与者进行了探诊深度（PD）、临床附着丧失（CAL）、探诊出血（BOP）、牙龈指数（GI）和菌斑指数（PI）的检查。使用纸尖收集龈沟液，使用棉卷收集全唾液样本。使用酶联免疫吸附测定试剂盒对不同生物标志物的样本进行分析。分析了基质金属蛋白酶（MMP）-8、MMP-9、乳铁蛋白、胱抑素C、髓过氧化物酶（MPO）、血小板活化因子、组织蛋白酶B和I型胶原吡啶交联羧基末端肽的水平。唾液中的MPO和MMP-8水平与牙龈炎密切相关，Pearson相关系数分别为0.399和0.217。MMP-8的曲线下面积（AUC）最大，为0.814，其次是MPO和MMP-9，分别为0.793和0.777。GI和PI的临床参数显示出很强的相关性和较大的AUC值，而PD和CAL则不然。发现MMP-8和MPO对诊断牙龈炎有效。基于本研究结果的进一步调查可能会确定临床上有用的生物标志物，用于准确、早期检测牙龈炎。
