比较不同红细胞类型中无性和有性疟原虫发育阶段。

Comparative analysis of asexual and sexual stage Plasmodium falciparum development in different red blood cell types.

机构信息

Department of Immunology, Noguchi Memorial Institute for Medical Research, University of Ghana, Accra, Ghana.

West African Centre for Cell Biology of Infectious Pathogens, University of Ghana, Accra, Ghana.

出版信息

Malar J. 2020 Jun 5;19(1):200. doi: 10.1186/s12936-020-03275-9.

DOI:10.1186/s12936-020-03275-9

PMID:32503587

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7275330/

Abstract

BACKGROUND

Red blood cell (RBC) polymorphisms are suggested to influence the course of Plasmodium falciparum malaria. Whereas some variants have been found to be protective, others have been found to enhance parasite development. This study evaluated the effect of variant haemoglobin (Hb) and ABO blood groups on P. falciparum merozoite invasion, multiplication rates as well as gametocyte development.

METHODS

Approximately 2.5 mL of venous blood was collected from each participant. Flow cytometry was used to determine the in vitro merozoite invasion rates of NF54 parasites into the blood of 66 non-parasitaemic individuals with variant Hb genotypes (HbSS, HbSC) and blood groups (A, B, O), which were then compared with invasion into HbAA blood. The ex vivo asexual parasite multiplication and gametocyte production rates of parasites from 79 uncomplicated malaria patients with varying Hb genotypes (HbAS, HbAC and HbAA) were also estimated using microscopy.

RESULTS

Merozoite invasion rates were significantly reduced by about 50% in RBCs containing HbSS and HbSC relative to HbAA cells. The presence of blood group O and B reduced the invasion rates of HbSS by about 50% and 60%, respectively, relative to HbSC but the presence of blood group A removed the inhibitory effect of HbSS. The initial parasite densities in uncomplicated malaria patients with Hb genotypes HbAS and HbAC cells were similar but significantly lower than those with genotype HbAA. The ex vivo parasite multiplication rate, gametocytaemia and gametocyte conversion rates followed a similar trend but did not reach statistical significance (p > 0.05).

CONCLUSIONS

Parasite invasion rate into erythrocytes is dependent on both erythrocyte blood group antigen and haemoglobin genotype as blood group O and B provided protection via reduced merozoite invasion in RBCs containing HbSS relative to HbSC. Regardless of haemoglobin type, greater than 70% malaria patients had circulating ring stage parasites that differentiated into stage II gametocytes in 4 days.

摘要

背景

红细胞（RBC）多态性被认为会影响恶性疟原虫疟疾的病程。虽然已经发现一些变体具有保护作用，但其他变体则增强了寄生虫的发育。本研究评估了变体血红蛋白（Hb）和 ABO 血型对恶性疟原虫裂殖子入侵、增殖率以及配子体发育的影响。

方法

从每个参与者采集约 2.5 毫升静脉血。流式细胞术用于确定 NF54 寄生虫进入 66 名非寄生性个体血液中的体外裂殖子入侵率，这些个体具有变体 Hb 基因型（HbSS、HbSC）和血型（A、B、O），然后与 HbAA 血液中的入侵率进行比较。使用显微镜还估计了来自 79 名患有不同 Hb 基因型（HbAS、HbAC 和 HbAA）的非复杂性疟疾患者的寄生虫体外无性繁殖和配子体产生率。

结果

与 HbAA 细胞相比，含有 HbSS 和 HbSC 的 RBC 中的裂殖子入侵率显著降低了约 50%。血型 O 和 B 的存在使 HbSS 的入侵率相对于 HbSC 降低了约 50%和 60%，但血型 A 消除了 HbSS 的抑制作用。具有 HbAS 和 HbAC 细胞的非复杂性疟疾患者的初始寄生虫密度相似，但明显低于具有 HbAA 基因型的患者。体外寄生虫繁殖率、配子体血症和配子体转化率也呈现出相似的趋势，但未达到统计学意义（p>0.05）。