National R & D Center for Edible Fungus Processing Technology, Henan University, Kaifeng, 475004, China; Joint International Research Laboratory of Food & Medicine Resource Function, Henan Province, Henan University, Kaifeng, 475004, China.
National R & D Center for Edible Fungus Processing Technology, Henan University, Kaifeng, 475004, China; Kaifeng Key Laboratory of Functional Components in Health Food, Kaifeng, 475004, China.
Food Chem Toxicol. 2019 Dec;134:110806. doi: 10.1016/j.fct.2019.110806. Epub 2019 Sep 12.
Insulin resistance causes several adverse effects such as hypertension, diabetes and different aspects of cardiovascular diseases. Patrinia scabiosaefolia Fisch. ex Trev. is a traditional Chinese edible herbal, whose n-BuOH extract significantly increased glucose transportin 3T3-L1 adipocytes at the concentration of 12.5 μM. To determine its active constituent, its chemical components and bioactivities were investigated. Two compounds (1-2) could significantly improve insulin resistance in 3T3-L1 adipocytes at concentrations around 25.0 μM (P < 0.001). Compound 2 was more effective to lower the content of glucose content at 12.5 μM (P < 0.001). Compound 1 was a new compound identified by spectroscopic methods. Western-blot experiment demonstrated an upregulation of p-IRS-1, p-Akt, and GLUT4 induced by compounds 1 and 2. Hence, we speculated that compounds 1 and 2 could activate PI3K and Akt signaling by up-regulating of p-IRS-1 which resulted in the activation of PI3K before phosphorylating Akt, ultimately led to translocation of GLUT4. These events finally improve glucose transport. Our results may provide the scientific basis for the development and effective use of P. scabiosaefolia against type 2 diabetes.
胰岛素抵抗会引起多种不良反应，如高血压、糖尿病和心血管疾病的各个方面。苣荬菜是一种传统的中国药食同源植物，其正丁醇提取物在 12.5µM 的浓度下显著增加了 3T3-L1 脂肪细胞中的葡萄糖转运蛋白 3。为了确定其活性成分，对其化学成分和生物活性进行了研究。两种化合物（1-2）在 25.0µM 左右的浓度下能显著改善 3T3-L1 脂肪细胞的胰岛素抵抗（P<0.001）。化合物 2 在 12.5µM 时更有效地降低葡萄糖含量（P<0.001）。化合物 1 是通过光谱方法鉴定的新化合物。Western-blot 实验表明，化合物 1 和 2 能上调 p-IRS-1、p-Akt 和 GLUT4。因此，我们推测化合物 1 和 2 可以通过上调 p-IRS-1 来激活 PI3K 和 Akt 信号通路，从而导致 Akt 磷酸化之前 PI3K 的激活，最终导致 GLUT4 的易位。这些事件最终改善了葡萄糖的转运。我们的研究结果为苣荬菜在治疗 2 型糖尿病方面的开发和有效利用提供了科学依据。
