Council of Scientific and Industrial Research-Institute of Microbial Technology, Molecular Biology Division, Sector 39-A, Chandigarh 160036, India.
Council of Scientific and Industrial Research-Institute of Microbial Technology, Molecular Biology Division, Sector 39-A, Chandigarh 160036, India.
Genomics. 2020 Sep;112(5):3179-3190. doi: 10.1016/j.ygeno.2020.06.004. Epub 2020 Jun 3.
Klebsiella variicola is an emerging pathogen responsible for causing blood-stream infections, urinary and respiratory tract related diseases in humans. In this report, we describe the genome sequence data and phenotypic characterization of K. variicola strain KV093 isolated from India. Comparative genome sequence analysis revealed the presence of genes linked with virulence, iron acquisition and transport, type 1 and type 3 pili, secretion systems including the capsular gene cluster. The plant-associated genes such as nitrogen fixation, growth and defense mechanisms against oxidative stress were also identified. On performing antibiotic susceptibility testing, growth inhibition, and stress challenge assays it was observed that the drug resistant K. variicola KV093 exhibited cross resistance to various antibiotics, antiseptics, including disinfectants. This report highlights the arsenal of virulence and antibiotic resistance determinants in K. variicola KV093, an effort emphasizing the current pressing need for regular surveillance of K. variicola strains especially in India.
产酸克雷伯菌是一种新兴的病原体，可导致人类血流感染、尿路感染和呼吸道相关疾病。在本报告中，我们描述了从印度分离的产酸克雷伯菌菌株 KV093 的基因组序列数据和表型特征。比较基因组序列分析显示，存在与毒力、铁获取和转运、1 型和 3 型菌毛、包括荚膜基因簇的分泌系统相关的基因。还鉴定了与氮固定、生长和防御氧化应激有关的植物相关基因。在进行抗生素药敏试验、生长抑制和应激挑战试验时，观察到耐药性产酸克雷伯菌 KV093 对各种抗生素、防腐剂包括消毒剂表现出交叉耐药性。本报告强调了产酸克雷伯菌 KV093 中毒力和抗生素耐药决定因素的多样性，这突出强调了当前迫切需要定期监测印度特别是产酸克雷伯菌菌株的必要性。
