de Campos Tatiana Amabile, de Almeida Felipe Marques, de Almeida Ana Paula Cardoso, Nakamura-Silva Rafael, Oliveira-Silva Mariana, de Sousa Isabela Felix Alencar, Cerdeira Louise, Lincopan Nilton, Pappas Georgios Joannis, Pitondo-Silva André
Departamento de Biologia Celular, Instituto de Ciências Biológicas, Universidade de Brasília, Brasília, Brazil.
Programa de Pós-graduação em Biologia Microbiana, Universidade de Brasília, Brasília, Brazil.
Front Microbiol. 2021 Apr 16;12:604031. doi: 10.3389/fmicb.2021.604031. eCollection 2021.
is mainly associated with opportunistic infections and frequently identified as This misidentification implies a wrong epidemiology result as well as incorrect attribution to as the etiology of some severe infections. Recently, huge efforts have been made to study , however, the biological aspects of this species are still unclear. Here we characterized five strains initially identified as , with a Vitek-2 System and 16S rRNA sequencing. One-step multiplex polymerase chain reaction and Whole Genome Sequencing (WGS) identified them as Additionally, WGS analysis showed that all the strains are closely related with genomes, forming a clustered group, apart from and Multilocus sequence typing analysis showed four different sequence types (STs) among the strains and for two of them (Kv97 and Kv104) the same ST was assigned. All strains were multidrug-resistant (MDR) and three showed virulence phenotypes including invasion capacity to epithelial cells, and survival in human blood and serum. These results showed the emergence of new clones with pathogenic potential to colonize and cause infection in different tissues. These characteristics associated with MDR strains raise great concern for human health.
主要与机会性感染相关，并且经常被鉴定为 。这种错误鉴定意味着流行病学结果错误以及将某些严重感染的病因错误归因于 。最近，人们为研究 付出了巨大努力，然而，该物种的生物学特性仍不清楚。在这里，我们使用Vitek-2系统和16S rRNA测序对最初鉴定为 的五株 菌株进行了表征。一步多重聚合酶链反应和全基因组测序（WGS）将它们鉴定为 。此外，WGS分析表明，所有菌株都与 基因组密切相关，形成一个聚类群，与 和 不同。多位点序列分型分析显示菌株中有四种不同的序列类型（STs），其中两种（Kv97和Kv104）被指定为相同的ST。所有菌株均为多重耐药（MDR），其中三株表现出毒力表型，包括对上皮细胞的侵袭能力以及在人血液和血清中的存活能力。这些结果表明出现了具有在不同组织中定殖和引起感染的致病潜力的新 克隆。这些与MDR菌株相关的特征引起了对人类健康的极大关注。
