Sir John Walsh Research Institute, University of Otago, New Zealand.
Sir John Walsh Research Institute, University of Otago, New Zealand.
Dent Mater. 2020 Aug;36(8):1096-1107. doi: 10.1016/j.dental.2020.05.001. Epub 2020 Jun 3.
To develop a silver nanoparticle (AgNP) formulation for incorporation into glass ionomer cements (GICs) which minimises biofilm growth on restoration surfaces.
GICs, Fuji IX, Ketac Molar, and Riva Selfcure were modified with 6, 10 and 24 μg per GIC capsule of α-lipoic acid-capped AgNPs. Monoculture biofilms of Streptococcus mutans were cultured (72 h) on GIC specimens (n = 3) and biofilm accumulation was quantified using a viability stain with confocal laser scanning microscopy. Compression strength and flexural strength (CS & FS) were measured according to ISO 9917-1:2007 (n = 8, n = 25). GIC colour was measured at 0, 1, and 14 days following AgNP incorporation using a digital spectrophotometer. Silver release from AgNP-modified GIC specimens was monitored at 1, 3, 7 and 14 days using inductively coupled plasma-mass spectrometry.
AgNP-modified Fuji IX demonstrated the greatest reduction in biofilm accumulation, with 10 μg Ag/capsule inhibiting biofilm formation by 99%. Ketac Molar and Riva Selfcure required 24 μg Ag/capsule to achieve 78% biofilm reduction. AgNP-modified GICs demonstrated significantly higher CS and FS than sintered silver-containing GICs, and possessed equivalent or higher strength values when compared to unmodified GICs. The colour shades of AgNP-modified GICs were more comparable to VITA shades of non-modified GICs than were sintered silver-containing GICs. The silver (≥99.6%) remained within the GIC for at least two weeks following incorporation.
AgNP-modified GICs exhibited significant antibiofilm activity and retained mechanical properties equivalent or superior to non-modified GICs. AgNP-modified GICs could reduce bacterial colonisation on and around restorations thereby reducing restoration failure caused by secondary caries.
开发一种载银纳米粒子（AgNP）的制剂，将其掺入玻璃离子水门汀（GIC）中，以最大限度地减少修复体表面生物膜的生长。
用 α-硫辛酸封端的 AgNP 对 Fuji IX、Ketac Molar 和 Riva Selfcure 三种 GIC 胶囊进行改性，每胶囊 6、10 和 24μg。在 GIC 标本上培养（72 小时）变形链球菌的单培养物生物膜，并使用共焦激光扫描显微镜的活度染色定量生物膜积累。根据 ISO 9917-1：2007 测量压缩强度和弯曲强度（CS 和 FS）（n=8，n=25）。在掺入 AgNP 后 0、1 和 14 天，使用数字分光光度计测量 GIC 颜色。使用电感耦合等离子体质谱法监测 1、3、7 和 14 天内 AgNP 改性 GIC 标本的银释放情况。
AgNP 改性的 Fuji IX 显示出对生物膜积累的最大抑制作用，其中 10μgAg/胶囊可抑制生物膜形成达 99%。Ketac Molar 和 Riva Selfcure 需要 24μgAg/胶囊才能达到 78%的生物膜减少率。AgNP 改性的 GIC 具有比烧结含银 GIC 更高的 CS 和 FS，并且与未改性 GIC 相比具有相当或更高的强度值。与烧结含银 GIC 相比，AgNP 改性的 GIC 的颜色色调更接近未改性 GIC 的 VITA 色调。在掺入后至少两周内，银（≥99.6%）仍保留在 GIC 中。
AgNP 改性的 GIC 表现出显著的抗生物膜活性，并保持与未改性 GIC 相当或更高的机械性能。AgNP 改性的 GIC 可以减少修复体周围和周围的细菌定植，从而减少继发龋引起的修复体失败。
