You Han-Sol, Jang Young-Sun, Sathiyaseelan Anbazhagan, Ryu Su-Ji, Lee Ha-Yeon, Baek Jong-Suep
Department of Bio-Health Convergence, Kangwon National University, Chuncheon, 24341, Republic of Korea.
College of Pharmacy, Kangwon National University, Chuncheon, 24341, Republic of Korea.
Int J Nanomedicine. 2025 Jan 8;20:343-366. doi: 10.2147/IJN.S485722. eCollection 2025.
Multi-walled carbon nanotubes (MWCNTs) were used as carriers for silver nanoparticles (AgNPs). In this process, MWCNTs were coated with mesoporous silica (MWCNT-Silica) for uniform and regular loading of AgNPs on the MWCNTs. In addition, astaxanthin (AST) extract was used as a reducing agent for silver ions to enhance the antioxidant, antibiofilm, and anticancer activities of AgNPs. In this process, AST was extracted from and processed by hot melt extrusion (HME) to enhance the AST content of . AST has strong antioxidative properties, which leads to anticancer activity. In addition, AgNPs are well known for their strong antibacterial properties. The antibiofilm and anticancer effects were studied comprehensively by loading the AST AgNPs onto MWCNT-Silica.
AgNPs-loaded MWCNT-silica (MWCNT-Ag) was prepared through the binding reaction of TSD and silanol groups and the aggregation interaction of Ag and TSD. To enhance the antioxidant, antibiofilm, and anticancer activities of AgNPs, HME-treated extract (HME-AST) was used as a reducing solution of silver ions. The increased AST content of HME-AST was confirmed by high-performance liquid chromatography (HPLC) analysis, and the total phenol and flavonoid content analysis confirmed that HME enhanced the active components of . The antibiofilm activity of MWCNT-AST was investigated by biofilm inhibition and destruction test, SEM, and CLSM analysis, and the anticancer activity was investigated by WST assay, fluorescent staining analysis, and flow cytometry analysis.
MWCNT-AST showed higher antioxidant activity and antibiofilm activity than MWCNT-Ag against , and methicillin-resistant (MRSA). MWCNT-AST showed higher anticancer activity against breast cancer cells (MDA-MB-231) than MWCNT-Ag, and lower toxicity in normal cells HaCaT and NIH3T3.
MWCNT-AST exhibits higher antioxidant, antibiofilm, and anticancer activities than MWCNT-Ag, and exhibits lower toxicity to normal cells.
多壁碳纳米管（MWCNTs）被用作银纳米颗粒（AgNPs）的载体。在此过程中，多壁碳纳米管被介孔二氧化硅包覆（MWCNT-二氧化硅），以使银纳米颗粒在多壁碳纳米管上均匀且规则地负载。此外，虾青素（AST）提取物被用作银离子的还原剂，以增强银纳米颗粒的抗氧化、抗生物膜和抗癌活性。在此过程中，虾青素从[具体来源]中提取并通过热熔挤出（HME）进行处理，以提高[具体材料]中的虾青素含量。虾青素具有很强的抗氧化性能，从而导致抗癌活性。此外，银纳米颗粒以其强大的抗菌性能而闻名。通过将AST-AgNPs负载到MWCNT-二氧化硅上，全面研究了其抗生物膜和抗癌作用。
通过TSD与硅醇基团的结合反应以及Ag与TSD的聚集相互作用制备负载AgNPs的MWCNT-二氧化硅（MWCNT-Ag）。为增强银纳米颗粒的抗氧化、抗生物膜和抗癌活性，将经HME处理的[具体提取物]（HME-AST）用作银离子的还原溶液。通过高效液相色谱（HPLC）分析确认HME-AST中虾青素含量增加，总酚和黄酮含量分析证实HME增强了[具体材料]的活性成分。通过生物膜抑制和破坏试验、扫描电子显微镜（SEM）和共聚焦激光扫描显微镜（CLSM）分析研究MWCNT-AST的抗生物膜活性，并通过WST测定、荧光染色分析和流式细胞术分析研究其抗癌活性。
MWCNT-AST对[具体细菌]和耐甲氧西林[具体细菌]（MRSA）显示出比MWCNT-Ag更高的抗氧化活性和抗生物膜活性。MWCNT-AST对乳腺癌细胞（MDA-MB-231）显示出比MWCNT-Ag更高的抗癌活性，并且对正常细胞HaCaT和NIH3T3的毒性更低。
MWCNT-AST比MWCNT-Ag表现出更高的抗氧化、抗生物膜和抗癌活性，并且对正常细胞表现出更低的毒性。
