Department of Biology, McMaster Univeristy, 1280 Main St. West, Hamilton, ON, L8S 4K1, Canada.
J Mol Evol. 2020 Aug;88(6):510-520. doi: 10.1007/s00239-020-09951-3. Epub 2020 Jun 6.
Gene expression in bacteria is a remarkably controlled and intricate process impacted by many factors. One such factor is the genomic position of a gene within a bacterial genome. Genes located near the origin of replication generally have a higher expression level, increased dosage, and are often more conserved than genes located farther from the origin of replication. The majority of the studies involved with these findings have only noted this phenomenon in a single gene or cluster of genes that was re-located to pre-determined positions within a bacterial genome. In this work, we look at the overall expression levels from eleven bacterial data sets from Escherichia coli, Bacillus subtilis, Streptomyces, and Sinorhizobium meliloti. We have confirmed that gene expression tends to decrease when moving away from the origin of replication in majority of the replicons analysed in this study. This study sheds light on the impact of genomic location on molecular trends such as gene expression and highlights the importance of accounting for spatial trends in bacterial molecular analysis.
细菌中的基因表达是一个受到多种因素影响的高度受控和复杂的过程。其中一个因素是基因在细菌基因组中的基因组位置。通常，位于复制起点附近的基因的表达水平更高，剂量增加，并且比位于复制起点较远的基因更保守。这些发现的大多数研究仅在单个基因或一组基因中注意到了这种现象，这些基因被重新定位到细菌基因组内的预定位置。在这项工作中，我们研究了来自大肠杆菌、枯草芽孢杆菌、链霉菌和苜蓿中华根瘤菌的 11 个细菌数据集的整体表达水平。我们已经证实，在本研究分析的大多数复制子中，随着远离复制起点，基因表达往往会降低。这项研究揭示了基因组位置对基因表达等分子趋势的影响，并强调了在细菌分子分析中考虑空间趋势的重要性。
