从孔鳐软骨中提取的硫酸软骨素的纯化、表征和抗氧化活性。
Purification, characterisation and antioxidant activities of chondroitin sulphate extracted from Raja porosa cartilage.
机构信息
College of Food Science and Technology, Hebei Agricultural University, Baoding, China.
College of Food Science and Technology, Hebei Agricultural University, Baoding, China; School of Medical Instrument and Food Engineering, University of Shanghai for Science and Technology, Shanghai, China.
出版信息
Carbohydr Polym. 2020 Aug 1;241:116306. doi: 10.1016/j.carbpol.2020.116306. Epub 2020 Apr 27.
This study was designed to isolate (isolation yield of 36.51 %) and characterise chondroitin sulphate (CS) from skate (Raja porosa) cartilage. Gel permeation chromatography demonstrated that the Raja porosa chondroitin sulphate (RPCS) obtained was a relatively uniform polysaccharide with a molecular weight of 40,752 Da and a purity of 94.0 %. Fourier-transform infrared, nuclear magnetic resonance spectroscopy and strong anion-exchange high-performance liquid chromatography indicated that the content of the predominant GlcA-GalNAc6S unit in RPCS was 65.84 %, which was higher than that in shark CS (53.93 %). Furthermore, RPCS displayed more effective free radical scavenging effects than did shark CS, indicating the potential of RPCS to promote oxidative stress resistance and to act as an antioxidant agent. Skate cartilage could be exploited as a sufficient and sustainable source for the preparation of CS with higher 6S-sulphation, which in turn could be scaled up for use in the pharmaceutical industry.
本研究旨在从软骨鱼（孔鳐）软骨中分离（分离产率为 36.51%）并表征硫酸软骨素（CS）。凝胶渗透色谱法表明，所得到的孔鳐硫酸软骨素（RPCS）是一种相对均匀的多糖，分子量为 40752 Da，纯度为 94.0%。傅里叶变换红外、核磁共振波谱和强阴离子交换高效液相色谱表明，RPCS 中主要的 GlcA-GalNAc6S 单元的含量为 65.84%，高于鲨鱼 CS（53.93%）。此外，RPCS 表现出比鲨鱼 CS 更强的自由基清除效果，表明 RPCS 具有促进抗氧化应激和作为抗氧化剂的潜力。软骨鱼可以作为制备具有更高 6S-硫酸化程度 CS 的充足且可持续的来源，从而可以扩大其在制药行业的应用。
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