Panda Abikshyeet, Behura Shyam Sundar, Bhuyan Lipsa, Dash Kailash Chandra, Kumar Vijay, Mukherjee Shirsha
Department of Oral Pathology and Microbiology, Kalinga Institute of Dental Sciences, Kalinga Institute of Industrial Technology Deemed to be University, Bhubaneswar, Odisha, India.
J Oral Maxillofac Pathol. 2020 Jan-Apr;24(1):183. doi: 10.4103/jomfp.JOMFP_214_19. Epub 2020 May 8.
Pathology involving the oral epithelium may alter the level of salivary concentration of LDH. Thus its estimation can be used as a non invasive screening tool for the early detection of OPMDs and also to predict its malignant transformation especially in high risk population.
To evaluate the salivary and serum levels of lactate dehydrogenase (LDH) in patients having of oral submucous fibrosis (OSMF) and leukoplakia and compare it with healthy individuals.
A total of 120 subjects were selected and divided into three groups comprising clinically diagnosed cases of OSMF and leukoplakia and healthy subjects as controls. Unstimulated whole saliva and blood samples were collected under aseptic conditions for biochemical estimation of LDH by Semiautomatic Analyzer using LDH kit utilizing enzymatic UV-Kinetic method. The values obtained were statistically analyzed using the SPSS software version 20.0. P-value < 0.05 was considered significant.
The mean salivary LDH level in Group I (OSMF) was 631.67 + 7.67, Group II (Leukoplakia) was 492.28 + 16.17 and Group III (Healthy Control) was 140.62 + 8.87. There was a statistically significant difference between the Serum and salivary LDH levels among the various groups of study population. A positive correlation between salivary LDH and serum LDH level was seen and the regression equation for OSMF and leukoplakia was computed.
A significant difference was found between mean salivary LDH Levels and serum LDH levels in patients with leukoplakia, OSMF and health controls. A positive correlation was also established between salivary and serum LDH levels in patients with OSMF and leukoplakia patients making saliva a potent non invasive tool for early prediction and detection of PMOD and its malignant transformation.
涉及口腔上皮的病理学改变可能会改变唾液中乳酸脱氢酶（LDH）的浓度水平。因此，对其进行评估可作为一种非侵入性筛查工具，用于早期检测口腔潜在恶性疾病（OPMDs），还可用于预测其恶性转化，尤其是在高危人群中。
评估口腔黏膜下纤维化（OSMF）和白斑患者唾液及血清中乳酸脱氢酶（LDH）的水平，并与健康个体进行比较。
共选取120名受试者，分为三组，包括临床诊断的OSMF和白斑病例以及作为对照的健康受试者。在无菌条件下收集非刺激性全唾液和血液样本，使用LDH试剂盒，通过半自动分析仪采用酶促紫外动力学方法对LDH进行生化检测。使用SPSS 20.0软件对所得数据进行统计学分析。P值<0.05被认为具有统计学意义。
第一组（OSMF）唾液LDH平均水平为631.67 + 7.67，第二组（白斑）为492.28 + 16.17，第三组（健康对照）为140.62 + 8.87。不同研究人群组之间血清和唾液LDH水平存在统计学显著差异。观察到唾液LDH与血清LDH水平呈正相关，并计算了OSMF和白斑的回归方程。
白斑、OSMF患者与健康对照者的唾液LDH平均水平和血清LDH水平之间存在显著差异。OSMF和白斑患者的唾液和血清LDH水平之间也建立了正相关，这使得唾液成为早期预测和检测PMOD及其恶性转化的有效非侵入性工具。
