Goku Phoebe Esinam, Orman Emmanuel, Quartey Anna Naa Kwarley, Ansong George Twum, Asare-Gyan Elsam Baffour
Department of Pharmaceutical Sciences, School of Pharmacy, Central University, Accra 23321, Ghana.
Department of Pharmaceutical Chemistry, School of Pharmacy, University of Health and Allied Sciences, Ho 23321, Ghana.
Evid Based Complement Alternat Med. 2020 May 17;2020:9717304. doi: 10.1155/2020/9717304. eCollection 2020.
This study was conducted to comparatively assess the anthelminthic activity of leaves, stem bark, and seeds of in order to identify which of the plant parts possess the highest anthelminthic activity. Three concentrations of ethanolic and hydroethanolic extracts of the plant parts (1 mg/ml, 2.5 mg/ml, and 5 mg/ml) were prepared and tested against using albendazole as the positive control and 0.9% normal saline solution as the negative control. Preliminary phytochemical investigation showed the presence of alkaloids, saponins, and reducing sugars of glycosides present in all the crude extracts of . Tannins were observed only in extracts of the leaves, while fixed oils were only present in the extracts of the seeds. The results of the anthelminthic activity testing indicated that all crude extracts prepared were more effective than albendazole in reducing paralysis time ( < 0.0001) and death time ( < 0.0001). It was further shown that the extracts from the seeds (SE and SHE) were more effective than the extracts from the stem bark and leaves both in reducing paralysis and death times. Fractionation of SE provided a fraction, SE, which was more effective than SE both in reducing paralysis and death times ( < 0.0001) and was established to contain fixed oils. The outcome of the current study has provided a scientific justification for the preference of the seeds of for the treatment of helminth infections and has shown that the fixed oils present in the seeds could be responsible for such activity.
本研究旨在比较评估[植物名称]叶、茎皮和种子的驱虫活性，以确定该植物的哪个部位具有最高的驱虫活性。制备了该植物部位的三种浓度的乙醇提取物和水乙醇提取物（1毫克/毫升、2.5毫克/毫升和5毫克/毫升），并以阿苯达唑作为阳性对照，0.9%生理盐水溶液作为阴性对照，对[寄生虫名称]进行测试。初步植物化学研究表明，[植物名称]所有粗提物中均存在生物碱、皂苷和糖苷类还原糖。仅在叶提取物中观察到单宁，而固定油仅存在于种子提取物中。驱虫活性测试结果表明，所制备的所有粗提物在缩短麻痹时间（P<0.0001）和死亡时间（P<0.0001）方面比阿苯达唑更有效。进一步表明，种子提取物（SE和SHE）在缩短麻痹和死亡时间方面比茎皮和叶提取物更有效。对SE进行分离得到一个组分SE，其在缩短麻痹和死亡时间方面比SE更有效（P<0.0001），并确定含有固定油。本研究结果为优先选用[植物名称]种子治疗蠕虫感染提供了科学依据，并表明种子中存在的固定油可能是这种活性的原因。
