Zhang Xinrong, Zhang Lei, Cui Lihong, Chen Ming, Liu Dongying, Tian Jingjing
Department of Digestive Medicine, Tianjin Nankai Hospital Tianjin, China.
Department of Chinese Medicine, Geriatric Hospital of Tianjin Municipal Civil Affairs Bureau Tianjin, China.
Int J Clin Exp Pathol. 2020 May 1;13(5):1017-1023. eCollection 2020.
Emerging evidence indicates that circular RNAs (circRNAs) are a novel class of biomarkers and therapeutic targets in malignancies. Circ_0026344 has been reported to be downregulated in colorectal cancer, and associated with prognosis. However, little is currently known regarding its expression and clinical significance in gastric cancer. In this study, we evaluated the expression level of circ_0026344 in two previous circRNAs chips (GSE78092 and GSE89143) for gastric cancer. 93 pairs of gastric cancer and adjacent non-tumour tissues were collected, and qRT-PCR was applied to determine circ_0026344 expression. The level of circ_0026344 in gastric cancer cells (MKN45 and AGS) and a normal gastric epithelial cell line (GES-1) was also analyzed. Chi-square test was used to identify the association between circ_0026344 level and clinicopathologic factors. Kaplan-Meier method with log-rank test was applied to compare survival curves. Cox regression analyses were used to assess the prognostic value of circ_0026344. In GSE24549 and GSE24550 datasets, downregulation of circ_0026344 was observed. In 93 cases of gastric cancer, circ_0026344 in cancer tissues was significantly decreased compared to adjacent non-cancerous tissues, and its level was markedly associated with tumour size, lymph node metastasis, TNM stage, and invasive depth. Furthermore, patients with lower expression of circ_0026344 showed significantly worse overall survival than those with higher expression. Additional, circ_0026344 expression was an independent predictor for overall survival. Summarily, our study highlights that downregulation of circ_0026344 is associated with tumour malignant behavior and it is a potential biomarker for gastric cancer prognosis.
新出现的证据表明，环状RNA（circRNAs）是恶性肿瘤中的一类新型生物标志物和治疗靶点。据报道，Circ_0026344在结直肠癌中表达下调，并与预后相关。然而，目前关于其在胃癌中的表达及临床意义知之甚少。在本研究中，我们评估了circ_0026344在两个先前的胃癌环状RNA芯片（GSE78092和GSE89143）中的表达水平。收集了93对胃癌组织和相邻的非肿瘤组织，并应用qRT-PCR检测circ_0026344的表达。还分析了胃癌细胞（MKN45和AGS）和正常胃上皮细胞系（GES-1）中circ_0026344的水平。采用卡方检验确定circ_0026344水平与临床病理因素之间的关联。应用Kaplan-Meier法和对数秩检验比较生存曲线。采用Cox回归分析评估circ_0026344的预后价值。在GSE24549和GSE24550数据集中，观察到circ_0026344表达下调。在93例胃癌中，癌组织中的circ_002634比相邻的非癌组织显著降低，其水平与肿瘤大小、淋巴结转移、TNM分期和浸润深度显著相关。此外，circ_0026344表达较低的患者总生存期明显低于表达较高的患者。另外，circ_0026344表达是总生存期的独立预测因子。总之，我们的研究强调circ_0026344的下调与肿瘤恶性行为相关，它是胃癌预后的潜在生物标志物。
