Department of Pediatric Nephrology, Children's Medical Center, The Second Xiangya Hospital, Central South University, No. 139 Middle Renmin Road, Changsha, Hunan 410011, China.
Biomed Res Int. 2020 May 17;2020:8587923. doi: 10.1155/2020/8587923. eCollection 2020.
Our study is aimed at investigating whether Lipopolysaccharide- (LPS-) treated podocytes could polarize naive CD4 T cells into different subsets in vitro.
Podocytes and mouse bone marrow-derived dendritic cells (BMDCs) were first cultured with 25 g/ml LPS for 6 hours, respectively. Then, naive CD4 T cells were cocultured with the LPS-treated podocytes or BMDCs at a ratio of 1 : 1 or 1 : 1 : 1. After 48 hours, we collected the suspended cells and supernatant from all groups to measure T helper (Th)17 cells, regulatory T (Treg) cells, and cytokine concentration.
We observed the expression of CD80 and major histocompatibility complex class II molecule (MHC II) in podocytes but did not found the upregulation of them after treating podocytes with LPS. LPS-treated podocytes could induce naive CD4 T cells to Th17 cells and Treg cells with a higher ratio of Th17/Treg than BMDCs. Possible interaction between podocytes and BMDCs may exist in the induction process of Th17 cells and Treg cells.
Our study proved that CD80 and MHC II were constitutively expressed in podocytes but not upregulated by LPS. LPS-treated podocytes could polarize naive CD4 T cells into Th17 and Treg cells and affect the Th17/Treg balance and may incline to cause a Th17 response.
本研究旨在探讨脂多糖（LPS）处理的足细胞是否能在体外将幼稚 CD4 T 细胞极化成为不同的亚群。
首先分别将足细胞和小鼠骨髓来源的树突状细胞（BMDCs）与 25μg/ml LPS 孵育 6 小时。然后，将幼稚 CD4 T 细胞与 LPS 处理的足细胞或 BMDCs 以 1:1 或 1:1:1 的比例共培养 48 小时后，收集所有组别的悬浮细胞和上清液，以测量 Th17 细胞、调节性 T（Treg）细胞和细胞因子浓度。
我们观察到足细胞表达 CD80 和主要组织相容性复合体 II 分子（MHC II），但用 LPS 处理后并未发现它们的上调。LPS 处理的足细胞可以诱导幼稚 CD4 T 细胞向 Th17 细胞和 Treg 细胞分化，且 Th17/Treg 比值高于 BMDCs。在 Th17 细胞和 Treg 细胞的诱导过程中，可能存在足细胞和 BMDCs 之间的相互作用。
本研究证明 CD80 和 MHC II 在足细胞中持续表达，而不受 LPS 上调。LPS 处理的足细胞可将幼稚 CD4 T 细胞极化为 Th17 和 Treg 细胞，并影响 Th17/Treg 平衡，可能倾向于引起 Th17 反应。
