School of Municipal and Environmental Engineering, Shandong Jianzhu University, JiNan, 250101, China.
Co-Innovation Center of Green Building, JiNan, 250101, China.
Sci Rep. 2020 Jun 8;10(1):9188. doi: 10.1038/s41598-020-66169-5.
Agricultural wastes, such as wheat bran and swine wastewater, were used for bioremediation of oil-contaminated soil. Two optimised strains that could degrade oil efficiently were selected. The result showed that the best ratio of strain A to strain B was 7:3. Swine wastewater could be a replacement for nitrogen source and process water for bioremediation. Next, the Box-Behnken design was used to optimise the culture medium, and the optimal medium was as follows: microbial dosage of 97 mL/kg, wheat bran of 158 g/kg and swine wastewater of 232 mL/kg. Under the optimal medium, the oil degradation rate reached 68.27 ± 0.71% after 40 d. The urease, catalase, and dehydrogenase activities in oil-contaminated soil all increased, and the microbe quantity increased significantly with manual composting. These investigations might lay a foundation for reducing the pollution of agricultural wastes, exploring a late model for bioremediation of oil-contaminated soil.
农业废弃物，如麦麸和猪废水，被用于生物修复受污染的土壤。选择了两种可以有效降解石油的优化菌株。结果表明，菌株 A 与菌株 B 的最佳比例为 7:3。猪废水可以替代氮源和生物修复的工艺用水。接下来，采用 Box-Behnken 设计对培养基进行了优化，最佳培养基为：微生物用量 97 mL/kg、麦麸用量 158 g/kg、猪废水用量 232 mL/kg。在最佳培养基条件下，40 d 后油的降解率达到 68.27 ± 0.71%。受污染土壤中的脲酶、过氧化氢酶和脱氢酶活性均有所提高，微生物数量随着人工堆肥显著增加。这些研究可能为减少农业废弃物污染、探索受污染土壤生物修复新模式奠定基础。
