Jarošová J, Šnábel V, Cavallero S, Chovancová G, Hurníková Z, Antolová D
Institute of Parasitology SAS, Hlinkova 3, 040 01 Košice, Slovakia.
University of Rome, P. le Aldo Moro 5, 00185 Rome, Italy.
Helminthologia. 2020 May 23;57(2):120-128. doi: 10.2478/helm-2020-0022. eCollection 2020 Jun 1.
The mouse bile duct tapeworm , is a potentially zoonotic species with a wide variety of reported definitive hosts of rodent genera. In the present study the occurrence of in free-living small mammals in selected areas of Slovakia and the retrospective analysis of epidemiological data published in Slovakia were performed. was detected in two animal species, the common shrew () and the European hamster () of 186 small mammals examined from two ecosystems, urban and natural ecosystem of national park. No mention about the presence of this parasite in Slovakia in the past was found following a bibliographical search. Partial sequences of the nuclear paramyosin gene showed the shrew isolate placed in a subclade together with from Portugal, with high bootstrap value for its differentiation from the sister species . Similarly, the analysis of the nuclear ribosomal ITS region placed the hamster isolate in the cluster composed of from Australia, Spain and Portugal. The Slovak isolate was the most distinctive sample among available , differing in 1.4 - 1.9% of nucleotides from the remaining isolates. The difference (seven of 17 nucleotide positions) was partially due to indel polymorphisms associated with two and five nucleotides. To our knowledge, these are the first reports of in Central Europe and also the first record of infection in the common shrew. A recently indicated zoonotic potential of along with a possibility of its direct transmission between animals and/or humans without the need of intermediate hosts pose a public health concern in contaminated areas of Slovakia. The use of molecular techniques may substantially facilitate more thorough understanding of the epidemiological situation of and related tapeworms in various ecosystems of the country.
小鼠胆管绦虫是一种具有潜在人畜共患性的物种，有多种啮齿类动物被报道为其终末宿主。在本研究中，对斯洛伐克选定地区自由生活的小型哺乳动物中该绦虫的出现情况进行了调查，并对斯洛伐克发表的流行病学数据进行了回顾性分析。在从城市和国家公园自然生态系统这两个生态系统中检查的186只小型哺乳动物的两种动物物种中检测到了该绦虫，即普通鼩鼱（）和欧洲仓鼠（）。通过文献检索，未发现过去斯洛伐克有关于这种寄生虫存在的报道。核副肌球蛋白基因的部分序列显示，鼩鼱分离株与来自葡萄牙的分离株一起置于一个亚分支中，与姐妹物种的分化具有较高的自展值。同样，核糖体ITS区域的分析将仓鼠分离株置于由来自澳大利亚、西班牙和葡萄牙的分离株组成的簇中。斯洛伐克分离株是现有分离株中最独特的样本，与其余分离株在1.4 - 1.9%的核苷酸上存在差异。这种差异（17个核苷酸位置中的7个）部分是由于与两个和五个核苷酸相关的插入缺失多态性。据我们所知，这些是中欧关于该绦虫的首次报道，也是普通鼩鼱感染的首次记录。最近表明的该绦虫的人畜共患潜力以及其在动物和/或人类之间无需中间宿主直接传播的可能性，在斯洛伐克受污染地区引起了公共卫生关注。分子技术的应用可能极大地有助于更全面地了解该国各种生态系统中该绦虫及相关绦虫的流行病学情况。
