Liu Xiao, Qiu Yu, Li Bin, Li Zhiqiang, Zhang Ying, Wang Jing, Xiong Qingqing
National-Local Joint Engineering Laboratory for Energy Conservation in Chemical Process Integration and Resources Utilization, Tianjin Key Laboratory of Chemical Process Safety, School of Chemical Engineering and Technology, Hebei University of Technology, GuangRong Dao 8, Hongqiao District, Tianjin 300130, PR China.
College of Chemical Engineering, Zhejiang University of Technology, Hangzhou 310014, PR China.
Spectrochim Acta A Mol Biomol Spectrosc. 2020 Oct 15;240:118541. doi: 10.1016/j.saa.2020.118541. Epub 2020 May 28.
Since the outbreak of anthrax attack in the United States in 2001, much effort has been done to detect anthrax spores. In this paper, a simple and facile lanthanide-containing ratiometric luminescence "turn on" probe is constructed to achieve sensitive and selective detection of anthrax spores biomarker - pyridine-2,6-carboxylic acid (DPA) by simply mixing Tb, sodium polyacrylate as well as red internal reference which could exclude some disturbance from the luminescence of biological tissue. Our probe shows remarkable luminescence enhancement upon addition of DPA. The detection limit for DPA is measured as 35.8 nM with a wide work range of 0-66.7 μM. Besides, our probe enables distinguish DPA selectively from numerous interfering materials in aqueous solution. Further test strip experiments demonstrate the potential practical application of our probe in DPA detection.
自2001年美国发生炭疽袭击事件以来，人们为检测炭疽孢子付出了诸多努力。本文构建了一种简单便捷的含镧系元素的比率发光“开启”探针，通过简单混合铽、聚丙烯酸钠以及红色内参物，实现对炭疽孢子生物标志物——吡啶 - 2,6 - 二羧酸（DPA）的灵敏且选择性检测，该内参物可排除生物组织发光带来的一些干扰。加入DPA后，我们的探针显示出显著的发光增强。DPA的检测限为35.8 nM，工作范围宽达0 - 66.7 μM。此外，我们的探针能够在水溶液中从众多干扰物质中选择性地分辨出DPA。进一步的试纸实验证明了我们的探针在DPA检测中的潜在实际应用价值。
