Department of Obstetrics and Gynecology, Universidade Federal de Minas Gerais, Belo Horizonte, Brazil.
ORIGEN - Centre for Reproductive Medicine, Belo Horizonte, Brazil.
Cryobiology. 2020 Aug;95:80-83. doi: 10.1016/j.cryobiol.2020.06.002. Epub 2020 Jun 10.
The aim of the study was to evaluate whether selecting embryos for transfer after prolonged culture after thaw (18-24 h) has better pregnancy rates than selecting embryos for transfer after short culture after thaw (2-5 h). We performed a double-blinded, randomized, controlled trial, evaluating 388 patients submitted to ART treatment who had embryos frozen on day-2 and subsequently transferred. All patients received the same endometrial priming with estradiol valerate followed by vaginal progesterone. Patients were randomized for Frozen embryo transfer 2-5 h after thaw (Group D2) or 18-24 h after thaw (Group D2/D3). The main Outcome Measure was ongoing pregnancy rate (OPR) at 20 weeks' gestation per embryo transfer. A total of 179 patients had embryos transferred 2-5 h after thaw and 209 patients had embryos transferred 18-24 h after thaw. The mean age in group D2 was 36 ± 4.4 and 36 ± 5.4 in group D2/D3. Ongoing pregnancy rate was 28% and 33.5% (p = 0.2) for groups D2 and D2/D3, respectively. These results suggest that increasing the culture time of embryos in one day to improve selection before transfer does not increase ongoing pregnancy rate. CLINICAL TRIAL REGISTRATION NUMBER: NCT03381001.
本研究旨在评估胚胎解冻后延长培养（18-24 小时）后进行移植与解冻后短时间培养（2-5 小时）后进行移植相比，是否具有更高的妊娠率。我们进行了一项双盲、随机、对照试验，评估了 388 名接受辅助生殖技术（ART）治疗的患者，这些患者的胚胎在第 2 天冷冻，随后进行了移植。所有患者均接受雌二醇戊酸联合阴道用黄体酮进行相同的子宫内膜准备。患者被随机分为解冻后 2-5 小时（D2 组）或 18-24 小时（D2/D3 组）进行冻融胚胎移植。主要观察指标为每个胚胎移植后的持续妊娠率（OPR）至 20 周妊娠。共有 179 名患者在解冻后 2-5 小时进行了胚胎移植，209 名患者在解冻后 18-24 小时进行了胚胎移植。D2 组的平均年龄为 36±4.4 岁，D2/D3 组为 36±5.4 岁。D2 组和 D2/D3 组的持续妊娠率分别为 28%和 33.5%（p=0.2）。这些结果表明，在一天内增加胚胎培养时间以改善移植前的选择并不能提高持续妊娠率。临床试验注册号：NCT03381001。
