Department of Obstetrics and Gynecology, CHA Fertility Center Gangnam, CHA University, Seoul, Korea.
J Korean Med Sci. 2022 Mar 28;37(12):e96. doi: 10.3346/jkms.2022.37.e96.
The single vitrified-warmed blastocyst transfer (SVBT) cycle has been increasingly utilized for assisted reproductive technology. Women of advanced maternal age (AMA) comprise a significant portion of patients who have undergone 'freeze-all' cycles. This study investigated the association between the post-warming extended culture duration and pregnancy outcomes in patients of AMA.
This retrospective cohort study analyzed the outcomes of 697 SVBT cycles between January 2016 and December 2017. The cycles were divided into 3 groups based on the age of the female partners: group I: < 35 years (n = 407), group II: 35-37 years (n = 176); and group III, 38-40 years (n = 114). Data are shown as the mean ± standard error of the mean. Data were analyzed using one-way ANOVA followed by Duncan's multiple range test. Statistical significance was set at < 0.001.
The blastocyst rate, clinical pregnancy rate, and live birth rate (LBR) was significantly lower in the AMA groups. However, there were no significant differences in LBR in the transfer between the AMA and younger groups according to blastocyst morphology and post-warming extended culture duration.
Post-warming extended culture of blastocysts is not harmful to patients of AMA. It could be a useful parameter in clinical counseling and decision making for fertility treatments.
单个玻璃化冷冻-解冻囊胚移植（SVBT）周期在辅助生殖技术中被越来越多地应用。高龄产妇（AMA）在经历“全部冷冻”周期的患者中占很大比例。本研究探讨了 AMA 患者囊胚解冻后延长培养时间与妊娠结局的关系。
本回顾性队列研究分析了 2016 年 1 月至 2017 年 12 月期间 697 例 SVBT 周期的结果。根据女性伴侣的年龄将周期分为 3 组：组 I：<35 岁（n=407）；组 II：35-37 岁（n=176）；组 III：38-40 岁（n=114）。数据以平均值±标准误差表示。采用单因素方差分析，随后采用 Duncan 多重范围检验进行数据分析。统计学意义设定为<0.001。
囊胚率、临床妊娠率和活产率（LBR）在 AMA 组显著降低。然而，根据囊胚形态和解冻后延长培养时间，在 AMA 组和年轻组之间，转移后的 LBR 没有显著差异。
囊胚解冻后延长培养对 AMA 患者无害。它可能是生育治疗临床咨询和决策的有用参数。
