Yaqub Atif, Malkani Naila, Shabbir Arifa, Ditta Sarwar Allah, Tanvir Fouzia, Ali Shaista, Naz Misbah, Kazmi Syed Akif Raza, Ullah Rehan
Department of Zoology, Government College University, Lahore, 54000, Pakistan.
Department of Chemistry, Government College University, Lahore, 54000, Pakistan.
Curr Microbiol. 2020 Sep;77(9):2287-2299. doi: 10.1007/s00284-020-02058-4. Epub 2020 Jun 13.
Copper nanoparticles (CuNPs), due to their cost-effective synthesis, interesting properties, and a wide range of applications in conductive inks, cooling fluids, biomedical field, and catalysis, have attracted the attention of scientific community in recent years. The aim of the present study was to develop and characterize antibacterial and anticancer CuNPs synthesized via chemical and biological methods, and further synthesize CuNPs conjugated with doxycycline to study their synergic effect. During the chemical synthesis, ascorbic acid was used as a stabilizing agent, while Zingiber officinale and Allium sativum-derived extracts were used during the biological methods for synthesis of CuNPs. Characterization of CuNPs was performed by transmission electron microscopy (TEM), UV-visible spectroscopy, Fourier transform infrared spectroscopy (FTIR), and X-ray crystallography (XRD). Antimicrobial evaluation of the nanomaterials against Pseudomonas aeruginosa and Escherichia coli was performed by using disk diffusion method, while anticancer behavior against HeLa and HepG2 cell lines was studied by MTT assay. TEM revealed spherical-shaped nanoparticles with mean size of 22.70 ± 5.67, 35.01 ± 5.84, and 19.02 ± 2.41 nm for CuNPs, Gin-CuNPs, and Gar-CuNPs, respectively, and surface plasmon resonance peaks were obtained at 570 nm, 575 nm, and 610 nm for CuNPs, Gar-CuNPs, and Gin-CuNPs, respectively. The results of FTIR confirmed the consumption of biomolecules from the plant extracts for the synthesis of CuNPs. XRD analysis also confirmed synthesis of CuNPs. Doxycycline-conjugated NPs exhibited more antibacterial effects than doxycycline or CuNPs alone. Copper nanoparticles prepared by biological synthesis are cost-effective and eco-friendly as compared to their chemical counterparts. The chemically synthesized nanoparticles displayed more significant antimicrobial activity when capped with doxycycline than Z. officinale and A. sativum-mediated CuNPs; however, green-synthesized nanoparticles showed greater anticancer activity than their chemical counterparts.
铜纳米颗粒（CuNPs）因其合成成本效益高、性能有趣且在导电油墨、冷却液、生物医学领域和催化等方面有广泛应用，近年来吸引了科学界的关注。本研究的目的是开发并表征通过化学和生物方法合成的抗菌和抗癌铜纳米颗粒，并进一步合成与多西环素共轭的铜纳米颗粒以研究其协同效应。在化学合成过程中，使用抗坏血酸作为稳定剂，而在生物合成方法中使用姜和大蒜提取物来合成铜纳米颗粒。通过透射电子显微镜（TEM）、紫外可见光谱、傅里叶变换红外光谱（FTIR）和X射线晶体学（XRD）对铜纳米颗粒进行表征。使用纸片扩散法对纳米材料针对铜绿假单胞菌和大肠杆菌进行抗菌评估，同时通过MTT法研究其对HeLa和HepG2细胞系的抗癌行为。TEM显示，铜纳米颗粒、姜-铜纳米颗粒（Gin-CuNPs）和蒜-铜纳米颗粒（Gar-CuNPs）的纳米颗粒呈球形，平均尺寸分别为22.70±5.67、35.01±5.84和19.02±2.41纳米，并且铜纳米颗粒、蒜-铜纳米颗粒和姜-铜纳米颗粒的表面等离子体共振峰分别在570纳米、575纳米和610纳米处获得。FTIR结果证实了植物提取物中的生物分子用于合成铜纳米颗粒。XRD分析也证实了铜纳米颗粒的合成。与单独的多西环素或铜纳米颗粒相比，多西环素共轭纳米颗粒表现出更强的抗菌作用。与化学合成的铜纳米颗粒相比，通过生物合成制备的铜纳米颗粒具有成本效益且环保。当用多西环素封端时，化学合成的纳米颗粒比姜和大蒜介导的铜纳米颗粒表现出更显著的抗菌活性；然而，绿色合成的纳米颗粒比其化学合成的对应物表现出更强的抗癌活性。
