Department of Pharmaceutical Technology, University of North Bengal, Raja Rammohunpur, Darjeeling, West Bengal, 734013, India.
Plant Biochemistry Laboratory, Department of Botany, University of North Bengal, Darjeeling, West Bengal, India.
Bioprocess Biosyst Eng. 2024 Aug;47(8):1151-1161. doi: 10.1007/s00449-024-02982-w. Epub 2024 Mar 27.
A considerable amount of fruit waste is being produced every day worldwide. The green synthesis of metal nanoparticles from fruit peel waste can be an innovative, cost-effective, and eco-friendly alternative to traditional methods. Copper nanoparticles (CuNPs) were synthesized by a green method using the pineapple peels extract (PLX) and copper sulfate pentahydrate. The formation of CuNPs was visually identified and detected by UV-Visible spectroscopy. The CuNPs were characterized by Fourier-transform infrared (FTIR) spectroscopy, particle size analyzer, scanning electron microscopy (SEM), and X-ray diffraction (XRD). The antioxidant and reducing power of CuNPs were conducted by %DPPH scavenging and electron transfer-based ferric reducing antioxidant power (FRAP) assay, respectively. The antibacterial properties of CuNPs were determined in gram-positive, and gram-negative bacteria. The results showed that the CuNPs were spherical in shape with mean particle size 290.5 nm. The zeta potential of the nanoparticles was found to be - 12.3 mV indicating the instability in the colloidal state. The FTIR study confirmed the peaks of phytochemicals present in the PLX and the nanoparticles supporting the use of pineapple peels as stabilizing, reducing and capping agents. Both the DPPH and reducing power assay depicted that the synthesized CuNPs had significant antioxidant activity. However, the synthesized CuNPs had strong inhibitory capacity against both gram-positive and gram-negative test organisms. Thus, the CuNPS could be used for its viable antibacterial potential to preserve fruits, flowers, and vegetables from bacterial contamination.
全球每天都会产生大量的水果废弃物。利用果皮废弃物进行金属纳米粒子的绿色合成，可以作为传统方法的一种创新、经济高效且环保的替代方法。本研究采用菠萝皮提取物（PLX）和五水硫酸铜通过绿色方法合成了铜纳米粒子（CuNPs）。通过紫外可见光谱法对 CuNPs 的形成进行了直观的识别和检测。采用傅里叶变换红外（FTIR）光谱、粒径分析仪、扫描电子显微镜（SEM）和 X 射线衍射（XRD）对 CuNPs 进行了表征。通过 %DPPH 清除和基于电子转移的铁还原抗氧化能力（FRAP）测定法分别对 CuNPs 的抗氧化和还原能力进行了研究。通过革兰氏阳性菌和革兰氏阴性菌来确定 CuNPs 的抗菌性能。结果表明，CuNPs 呈球形，平均粒径为 290.5nm。纳米粒子的 zeta 电位为-12.3mV，表明胶体状态不稳定。FTIR 研究证实了 PLX 中存在的植物化学物质的峰以及纳米粒子的存在，支持使用菠萝皮作为稳定、还原和封端剂。DPPH 和还原能力测定均表明，合成的 CuNPs 具有显著的抗氧化活性。然而，合成的 CuNPs 对革兰氏阳性和革兰氏阴性测试生物均具有很强的抑制能力。因此，CuNPS 可用于其可行的抗菌潜力，以防止水果、花卉和蔬菜受到细菌污染。
