Vegetable Genetics and Breeding Laboratory, College of Horticulture, Anhui Agricultural University, Hefei, 230036, China.
Anhui Provincial Engineering Laboratory of Horticultural Crop Breeding, Hefei, 230036, China.
BMC Genomics. 2020 Jun 15;21(1):401. doi: 10.1186/s12864-020-06802-9.
Proline-rich extension-like receptor protein kinases (PERKs) are an important class of receptor kinases located in the plasma membrane, most of which play a vital role in pollen development.
Our study identified 25 putative PERK genes from the whole Brassica rapa genome (AA). Phylogenetic analysis of PERK protein sequences from 16 Brassicaceae species divided them into four subfamilies. The biophysical properties of the BrPERKs were investigated. Gene duplication and synteny analyses and the calculation of Ka/Ks values suggested that all 80 orthologous/paralogous gene pairs between B. rapa and A. thaliana, B. nigra and B. oleracea have experienced strong purifying selection. RNA-Seq data and qRT-PCR analyses showed that several BrPERK genes were expressed in different tissues, while some BrPERKs exhibited high expression levels only in buds. Furthermore, comparative transcriptome analyses from six male-sterile lines of B. rapa indicated that 7 BrPERK genes were downregulated in all six male-sterile lines. Meanwhile, the interaction networks of the BrPERK genes were constructed and 13 PERK coexpressed genes were identified, most of which were downregulated in the male sterile buds.
Combined with interaction networks, coexpression and qRT-PCR analyses, these results demonstrated that two BrPERK genes, Bra001723.1 and Bra037558.1 (the orthologs of AtPERK6 (AT3G18810)), were downregulated beginning in the meiosis II period of male sterile lines and involved in anther development. Overall, this comprehensive analysis of some BrPERK genes elucidated their roles in male sterility.
富含脯氨酸延伸样受体蛋白激酶（PERKs）是一类位于质膜上的重要受体激酶，其中大多数在花粉发育中起着至关重要的作用。
我们从整个 Brassica rapa 基因组（AA）中鉴定出 25 个推定的 PERK 基因。来自 16 种芸薹属植物的 PERK 蛋白序列的系统发育分析将它们分为四个亚家族。研究了 BrPERKs 的生物物理特性。基因复制和同线性分析以及 Ka/Ks 值的计算表明，B. rapa 和 A. thaliana、B. nigra 和 B. oleracea 之间的 80 对同源/旁系基因对都经历了强烈的纯化选择。RNA-Seq 数据和 qRT-PCR 分析表明，几个 BrPERK 基因在不同组织中表达，而一些 BrPERKs 仅在芽中表达高水平。此外，来自 6 个甘蓝型油菜雄性不育系的比较转录组分析表明，6 个雄性不育系中均有 7 个 BrPERK 基因下调。同时，构建了 BrPERK 基因的互作网络，鉴定出 13 个 PERK 共表达基因，其中大多数在雄性不育芽中下调。
结合互作网络、共表达和 qRT-PCR 分析，这些结果表明，两个 BrPERK 基因 Bra001723.1 和 Bra037558.1（AtPERK6（AT3G18810）的同源基因）从雄性不育系减数分裂 II 期开始下调，参与花药发育。总体而言，对一些 BrPERK 基因的综合分析阐明了它们在雄性不育中的作用。
