Yu A, Watts H, Jaffe N, Parkman R
N Engl J Med. 1977 Jul 21;297(3):121-7. doi: 10.1056/NEJM197707212970301.
The lack of detectable tumor-specific cytotoxicity by the peripheral blood lymphocytes of patients with cancer may be due to a lack of cytotoxic lymphocytes or the presence of suppressor lymphocytes that inhibit cytotoxic cells. Unfractionated peripheral blood lymphocytes from 12 of 28 patients with osteogenic sarcoma were cytotoxic to osteogenic sarcoma cells in vitro (P less than 0,001). When the peripheral blood lymphocytes from patients whose lymphocytes were not cytotoxic underwent fractionation, a tumor-specific cytotoxic subpopulation was isolated from 11 of 13 patients (P less than 0.0001). Lymphocytes that inhibited cytotoxic activity of autologous tumor-specific cytotoxic lymphocytes were found in four of 10 patients with osteogenic sarcoma but not in six normal controls. Inhibitor lymphocytes form rosettes with sheep erythrocytes and adhere to nylon, whereas cytotoxic lymphocytes have a receptor for C3 but no surface immunoglobulin. The lack of tumor-specific lymphocytotoxicity in some patients can be due to inhibitor lymphocytes.
癌症患者外周血淋巴细胞缺乏可检测到的肿瘤特异性细胞毒性，可能是由于缺乏细胞毒性淋巴细胞，或者存在抑制细胞毒性细胞的抑制性淋巴细胞。28例骨肉瘤患者中有12例的未分离外周血淋巴细胞在体外对骨肉瘤细胞具有细胞毒性（P小于0.001）。当对淋巴细胞无细胞毒性的患者的外周血淋巴细胞进行分离时，13例患者中有11例分离出肿瘤特异性细胞毒性亚群（P小于0.0001）。在10例骨肉瘤患者中有4例发现了抑制自体肿瘤特异性细胞毒性淋巴细胞细胞毒性活性的淋巴细胞，但在6例正常对照中未发现。抑制性淋巴细胞与绵羊红细胞形成玫瑰花结并黏附于尼龙，而细胞毒性淋巴细胞具有C3受体但无表面免疫球蛋白。一些患者缺乏肿瘤特异性淋巴细胞毒性可能是由于抑制性淋巴细胞所致。
