Department of Forensic Medicine, Post Graduate Institute of Medical Education and Research (PGIMER), Chandigarh, India.
DNA Fingerprinting Unit, State Forensic Science Laboratory, Sagar, India.
Ann Hum Biol. 2020 Aug;47(5):483-489. doi: 10.1080/03014460.2020.1772877. Epub 2020 Jun 16.
Autosomal STR typing using capillary electrophoresis is a reliable method for establishing parentage and for deciphering genomic ancestry.
This study was planned to show the genetic diversity of the Jat Sikh population, which is a widespread community of the Punjab region, and to assess its genetic relationship with existing Indian populations.
Blood samples of unrelated healthy individuals of the Jat Sikhs (n = 123) were used in this study. Fifteen autosomal STR markers along with the sex determination genetic marker Amelogenin were amplified using AmpFSTRIdentifiler Plus kit, and genetic analyser 3100 was used for genotyping.
A total of 246 alleles were observed with allele frequencies ranging from 0.004 to 0.447. The heterozygosity ranged from 0.659 to 0.886, and all studied loci were in Hardy-Weinberg Equilibrium (HWE). Fibrinogen A alpha (Aα) chain (FGA) was found to be the most polymorphic and also the most discriminating locus in the studied population. Neighbor-joining (NJ) tree, principal component analysis (PCA) plot, and Nei's Distance matrix revealed genetic affinity with the previously reported Jatt Sikh (Punjab) population and showed the outlier nature of this population compared with other Indian populations.
The data generated by this study enhance the database of Indian populations to be used in civil and forensic cases and also in other population-based genetic studies.
使用毛细管电泳进行常染色体 STR 分型是建立亲子关系和解析基因组亲缘关系的可靠方法。
本研究旨在展示广泛分布于旁遮普地区的贾特锡克人群的遗传多样性，并评估其与现有印度人群的遗传关系。
本研究使用了 123 名无关健康的贾特锡克个体的血液样本。使用 AmpFSTRIdentifiler Plus 试剂盒扩增了 15 个常染色体 STR 标记和性别决定遗传标记 Amelogenin，并使用遗传分析仪 3100 进行基因分型。
共观察到 246 个等位基因，等位基因频率范围为 0.004 至 0.447。杂合度范围为 0.659 至 0.886，所有研究的位点均处于 Hardy-Weinberg 平衡（HWE）状态。纤维蛋白原 A 链（FGA）被发现是研究人群中最具多态性和最具区分力的位点。邻接（NJ）树、主成分分析（PCA）图和 Nei 的距离矩阵显示了与先前报道的贾特锡克（旁遮普）人群的遗传亲和力，并显示了该人群与其他印度人群相比的异常性质。
本研究产生的数据增强了印度人群的数据库，可用于民事和法医案件以及其他基于人群的遗传研究。
