Alharbi Sara F, Alamri Asim, Elshehawi Ahmed
Biotechnology, Taif University, Taif, SAU.
Pediatric Oncology, King Salman Medical City, Medina, SAU.
Cureus. 2022 Nov 1;14(11):e30954. doi: 10.7759/cureus.30954. eCollection 2022 Nov.
Short tandem repeats (STRs) have been used for various identity typing methods worldwide. They have high discrimination power in human identification in forensics, paternity testing, missed personal identification, genetic diseases, and gene regulatory functions. They have also been used to detect and monitor the stability of diseases, including various types of cancer. This study aimed to investigate the impact of leukemia on the detection and stability of STR markers.
DNA was isolated from 30 participants (15 with chronic myeloid leukemia( CML) and 15 healthy controls) and used to amplify STR markers using specific primers.
We found that the blood of those with leukemia had more 9.3 and 9 alleles at the tyrosine hydroxylase 1 (TH01) marker than the blood of the healthy control samples. The results of this study will help researchers understand leukemia's effect on the detection and stability of STR markers in leukemic patients compared to healthy individuals.
Our results demonstrate that STR markers could become useful in genetic studies of leukemia cases.
短串联重复序列(STRs)已在全球范围内用于各种身份鉴定方法。它们在法医鉴定、亲子鉴定、失踪人员身份鉴定、遗传疾病和基因调控功能的人类识别中具有很高的鉴别力。它们还被用于检测和监测包括各种癌症在内的疾病的稳定性。本研究旨在调查白血病对STR标记物检测和稳定性的影响。
从30名参与者(15名慢性髓性白血病(CML)患者和15名健康对照者)中分离DNA,并使用特异性引物扩增STR标记物。
我们发现,白血病患者血液中酪氨酸羟化酶1(TH01)标记物的9.3和9等位基因比健康对照样本的血液中更多。本研究结果将有助于研究人员了解与健康个体相比,白血病对白血病患者STR标记物检测和稳定性的影响。
我们的结果表明,STR标记物在白血病病例的遗传研究中可能会有用。