Defence Institute of Physiology and Allied Sciences, Defence Research and Development Organization, Ministry of Defence, Government of India, Lucknow Road, Delhi, 110054, India.
State Forensic Science Laboratory, Civil Lines, Sagar, Madhya Pradesh, 470001, India.
Sci Rep. 2016 Sep 1;6:32494. doi: 10.1038/srep32494.
Genotyping of highly polymorphic autosomal short tandem repeat (STR) markers is a potent tool for elucidating genetic diversity. In the present study, fifteen autosomal STR markers were analyzed in unrelated healthy male Gorkha individuals (n = 98) serving in the Indian Army by using AmpFlSTR Identifiler Plus PCR Amplification Kit. In total, 138 alleles were observed with corresponding allele frequencies ranging from 0.005 to 0.469. The studied loci were in Hardy-Weinberg Equilibrium (HWE). Heterozygosity ranged from 0.602 to 0.867. The most polymorphic locus was Fibrinogen Alpha (FGA) chain which was also the most discriminating locus as expected. Neighbor Joining (NJ) tree and principal component analysis (PCA) plot clustered the Gorkhas with those of Nepal and other Tibeto-Burman population while lowlander Indian population formed separate cluster substantiating the closeness of the Gorkhas with the Tibeto-Burman linguistic phyla. Furthermore, the dataset of STR markers obtained in the study presents a valuable information source of STR DNA profiles from personnel for usage in disaster victim identification in military exigencies and adds to the Indian database of military soldiers and military hospital repository.
常染色体短串联重复序列（STR）基因分型是阐明遗传多样性的有力工具。本研究采用 AmpFlSTR Identifiler Plus PCR 扩增试剂盒，对在印度军队服役的 98 名无亲缘关系的健康男性廓尔喀个体的 15 个常染色体 STR 标记进行了分析。共观察到 138 个等位基因，相应的等位基因频率范围为 0.005 至 0.469。研究的位点处于哈迪-温伯格平衡（HWE）中。杂合度范围为 0.602 至 0.867。多态性最高的位点是纤维蛋白原 Alpha（FGA）链，这也是预期最具区分度的位点。邻接聚类（NJ）树和主成分分析（PCA）图将廓尔喀人与尼泊尔和其他藏缅语人群聚类在一起，而低地印度人群则形成单独的聚类，这证实了廓尔喀人与藏缅语语系的密切关系。此外，本研究中获得的 STR 标记数据集为军事人员的 STR DNA 图谱提供了有价值的信息来源，可用于军事紧急情况下的灾难遇难者识别，并为印度军人数据库和军人医院存储库增添了内容。
