lncRNA-RP11-156p1.3 通过 CRISPR/Cas9 编辑在肝细胞癌中的新型诊断和治疗靶点。
lncRNA- RP11-156p1.3, novel diagnostic and therapeutic targeting via CRISPR/Cas9 editing in hepatocellular carcinoma.
机构信息
The department of medicinal biochemistry and molecular biology, The school of Medicine, University of Ain Shams, Egypt.
Department of General Surgery, the school of Medicine, University of Ain Shams, Abbassia, Cairo, Egypt.
出版信息
Genomics. 2020 Sep;112(5):3306-3314. doi: 10.1016/j.ygeno.2020.06.020. Epub 2020 Jun 13.
We aim to characterize the expression of RNA panel in HCC. We assessed the expression of HCC-associated mRNA, miRNA and lncRNA network by real time PCR in sera and tissue samples. In a proof-of-principle approach, CRISPR cas9 mediated knock out for lncRNA- RP11-156p1.3 was performed in HEPG2 cell line to validate the role of the chosen RNA in HCC pathogenesis. The differential expression of RFTN1 mRNA, lncRNA- RP11-156p1.3 and miRNA-4764-5p was statistically different among the studied groups. After CRISPR cas9 mediated knockout of lncRNA- RP11-156p1.3 in HEPG2 cells, there was significant decrease in cell count and viability with reversal of the expression of the chosen RNAs. The chosen RNAs play a significant role in HCC pathogenesis and may be potential diagnostic and therapeutic targets.
我们旨在描述 HCC 中 RNA 谱的表达。我们通过实时 PCR 评估了血清和组织样本中与 HCC 相关的 mRNA、miRNA 和 lncRNA 网络的表达。在原理验证方法中，在 HEPG2 细胞系中使用 CRISPR cas9 介导的敲除技术对 lncRNA-RP11-156p1.3 进行了敲除，以验证所选 RNA 在 HCC 发病机制中的作用。在研究的各组之间，RFTN1 mRNA、lncRNA-RP11-156p1.3 和 miRNA-4764-5p 的差异表达具有统计学意义。在 HEPG2 细胞中使用 CRISPR cas9 介导的 lncRNA-RP11-156p1.3 敲除后，细胞计数和活力明显下降，所选 RNA 的表达逆转。所选 RNA 在 HCC 发病机制中发挥重要作用，可能是潜在的诊断和治疗靶点。
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