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负载小干扰RNA的硒纳米颗粒通过Bax信号通路抑制肠道病毒71型

Inhibition of Enterovirus 71 by Selenium Nanoparticles Loaded with siRNA through Bax Signaling Pathways.

作者信息

Lin Zhengfang, Li Yinghua, Xu Tiantian, Guo Min, Wang Changbing, Zhao Mingqi, Chen Haiyang, Kuang Jianling, Li Wanling, Zhang Yingying, Lin Tao, Chen Yi, Chen Huanhui, Zhu Bing

机构信息

Center Laboratory, Guangzhou Women and Children's Medical Center, Guangzhou Medical University, No 318 Renminzhong Road, Yuexiu District, Guangzhou 510120, Guangdong, People's Republic of China.

出版信息

ACS Omega. 2020 May 13;5(21):12495-12500. doi: 10.1021/acsomega.0c01382. eCollection 2020 Jun 2.

DOI:10.1021/acsomega.0c01382
PMID:32548434
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7271353/
Abstract

Enterovirus 71 (EV71) is the principal pathogen leading to severe cases of hand, foot, and mouth disease (HFMD). Specific drugs for EV71 are not discovered currently. Small interfering RNA (siRNA) provides a promising antiviral treatment pathway, but it is difficult to cross cell membranes and is easy to degrade. Nanoparticles are promising for their carrying capacity currently. In this study, the siRNA targeting EV71 VP1 gene was loaded with selenium nanoparticles (SeNPs) and surface decorated with polyethylenimine (PEI) (Se@PEI@siRNA). Se@PEI@siRNA showed a remarkable interference efficiency in the nerve cell line SK-N-SH and prevented the cells to be infected. The mechanism study revealed that Se@PEI@siRNA could lighten the extent of SK-N-SH cells for staying in the sub-G1 phase. Activation of Bax apoptosis signaling was restrained either. Taken together, this study demonstrated that Se@PEI@siRNA is a promising drug against EV71 virus.

摘要

肠道病毒71型（EV71）是导致手足口病（HFMD）重症病例的主要病原体。目前尚未发现针对EV71的特效药物。小干扰RNA（siRNA）提供了一种有前景的抗病毒治疗途径，但它难以穿过细胞膜且容易降解。纳米颗粒因其目前的运载能力而颇具前景。在本研究中，靶向EV71 VP1基因的siRNA被装载到硒纳米颗粒（SeNPs）上，并在表面用聚乙烯亚胺（PEI）进行修饰（Se@PEI@siRNA）。Se@PEI@siRNA在神经细胞系SK-N-SH中显示出显著的干扰效率，并防止细胞被感染。机制研究表明，Se@PEI@siRNA可以减轻SK-N-SH细胞停留在G1期的程度。Bax凋亡信号的激活也受到抑制。综上所述，本研究表明Se@PEI@siRNA是一种有前景的抗EV71病毒药物。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6179/7271353/6b3598cee923/ao0c01382_0004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6179/7271353/76a8449458a4/ao0c01382_0001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6179/7271353/a8304972ca19/ao0c01382_0002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6179/7271353/7b99c02871bf/ao0c01382_0003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6179/7271353/6b3598cee923/ao0c01382_0004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6179/7271353/76a8449458a4/ao0c01382_0001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6179/7271353/a8304972ca19/ao0c01382_0002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6179/7271353/7b99c02871bf/ao0c01382_0003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6179/7271353/6b3598cee923/ao0c01382_0004.jpg

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