Nimsarkar Prajakta, Ingale Prajakta, Singh Shailza
National Centre for Cell Science, NCCS Complex, 568 Ganeshkhind, SP Pune University Campus, Pune 411007, India.
ACS Omega. 2020 May 18;5(21):12516-12526. doi: 10.1021/acsomega.0c01502. eCollection 2020 Jun 2.
Leishmaniasis, the second most neglected tropical disease, has been reported to affect approximately 12 million people worldwide. The causative protozoan parasite has shown drug resistance to available chemotherapies, owing to which we need to look for better approaches to deal with the clinical situations. As per recent reports, several miRNAs have been found to be differentially expressed during infection in host macrophages. We aim to evaluate the impact of miRNA-mediated gene regulation on the key players of inflammation and macrophage dysfunction. The origin of miRNAs and their processing is a questionable phenomenon as of yet. Through our study, we aim to provide a framework of their characterization. We amalgamate chemical systems biology and synthetic biology approaches to identify putative miRNA targets and unravel the complexity of host-pathogen gene regulatory networks.
利什曼病是第二大被忽视的热带病，据报道全球约有1200万人受其影响。致病原生动物寄生虫已对现有的化疗药物产生耐药性，因此我们需要寻找更好的方法来应对临床情况。根据最近的报道，已发现几种microRNA（miRNA）在宿主巨噬细胞感染期间差异表达。我们旨在评估miRNA介导的基因调控对炎症和巨噬细胞功能障碍关键参与者的影响。miRNA的起源及其加工至今仍是一个有疑问的现象。通过我们的研究，我们旨在提供一个对其进行表征的框架。我们将化学系统生物学和合成生物学方法结合起来，以识别假定的miRNA靶标，并揭示宿主-病原体基因调控网络的复杂性。
