Rochelle P A, Day M J, Fry J C
Department of Applied Biology, University of Wales Institute of Science and Technology, Cardiff, UK.
J Gen Microbiol. 1988 Nov;134(11):2933-41. doi: 10.1099/00221287-134-11-2933.
A 7.8 kb plasmid (pQM17) encoding mercury resistance was isolated from two epilithic strains of Acinetobacter calcoaceticus. The plasmid had a broad host range when mobilized by RP1, transferring into Pseudomonas aeruginosa, P. putida, P. fluorescens, Escherichia coli, Proteus vulgaris and Chromobacterium sp. with frequencies ranging from 5.3 x 10(-9) to 4.6 x 10(-4) per recipient. The plasmid could be transferred into A. calcoaceticus BD413 using intact cells of donor and recipient bacteria (i.e. natural transformation) and there was a broad temperature optimum (14-37 degrees C) for transformation. Transformation was as efficient in liquid matings as on plates but there was no effect of pH in the range 5.6-7.9. Maximum transformation frequencies were obtained after 24 h on agar plates containing 3.5-10 g C 1-1 with donor to recipient ratios ranging from 6 to 415.
从两株石栖性醋酸钙不动杆菌中分离出一个编码汞抗性的7.8 kb质粒（pQM17）。当由RP1介导转移时，该质粒具有广泛的宿主范围，可转移到铜绿假单胞菌、恶臭假单胞菌、荧光假单胞菌、大肠杆菌、普通变形杆菌和色杆菌属中，转移频率为每个受体5.3×10⁻⁹至4.6×10⁻⁴。该质粒可以使用供体和受体细菌的完整细胞（即自然转化）转移到醋酸钙不动杆菌BD413中，并且转化具有较宽的最适温度范围（14 - 37℃）。液体交配中的转化效率与平板上相同，但在5.6 - 7.9的pH范围内没有影响。在含有3.5 - 10 g C⁻¹且供体与受体比例为6至415的琼脂平板上培养24小时后，可获得最大转化频率。
