不动杆菌属中克隆载体的转化与迁移
Transformation and mobilization of cloning vectors in Acinetobacter spp.
作者信息
Singer J T, van Tuijl J J, Finnerty W R
出版信息
J Bacteriol. 1986 Jan;165(1):301-3. doi: 10.1128/jb.165.1.301-303.1986.
Abstract
R300B-, RSF1010-, and RK2-derived plasmids were introduced into Acinetobacter sp. strain HO1-N and Acinetobacter calcoaceticus BD413 by transformation and conjugal mobilization. The transformation frequencies of BD413 were 4.2 X 10(6) to 6.3 X 10(6) transformants per micrograms of DNA per 10(9) recipient cells. Conjugal mobilization frequencies were 1.1 X 10(-1) to 8.5 X 10(-1) per recipient. An improved method for the transformation of A. calcoaceticus BD413 is reported.
摘要
通过转化和接合转移，将R300B -、RSF1010 -和RK2衍生的质粒导入不动杆菌属菌株HO1 - N和醋酸钙不动杆菌BD413。BD413的转化频率为每微克DNA每10⁹个受体细胞产生4.2×10⁶至6.3×10⁶个转化子。接合转移频率为每个受体1.1×10⁻¹至8.5×10⁻¹。报道了一种改进的醋酸钙不动杆菌BD413转化方法。
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