Abdel-Megeed Rehab M, El Newary Samah A, Kadry Mai O, Ghanem Hassan Z, El-Shesheny Rabeh A, Said-Al Ahl Hussein A H, Abdel-Hamid Abdel-Hamid Z
Therapeutic Chemistry Department, Pharmaceutical and Drug Industries Division, National Research Center, El Buhouth St., Dokki, Cairo, 12622 Egypt.
Medicinal and Aromatic Plants Researches Department, Pharmaceutical and Drug Industries Division, National Research Centre, El Buhouth St., Dokki, Cairo, Egypt.
J Diabetes Metab Disord. 2020 May 11;19(1):483-491. doi: 10.1007/s40200-020-00535-y. eCollection 2020 Jun.
Type 2 diabetes mellitus (DMT2) is contributed to dual interactions between environmental factors and certain genetic factors. This impressed a great need for novel treatment strategy. Nevertheless, ( as a terrestrial herb is considered to be an important source of natural antioxidants, it could be assessed as an anti-hyperglycemic agent.
In the current study, HPLC identified the active constitutes of , including total polyphenols, and flavonoids. Type 2 diabetes mellitus was induced in male Wistar albino rats a single dose of streptozotocin (STZ) (35 mg/kg BW). One week post diabetes induction, rats were administrated (500 mg/ kg BW) orally for one month. Molecular analysis was assessed to investigate the efficiency of on modulating ATP-binding cassette transporter A1 () and G1 () genes, in addition to apoptotic biomarkers, glycogen synthase kinase-3β () and cellular oncogene-fos () genes. Furthermore, inflammatory biomarkers, nuclear factor kappa-B () and tumor necrosis factor-α () gene expression were also assessed.
alcoholic extract declared the presence of polyphenols as gallic acid and flavonoids as quercetin in addition to many active constituents. Apigenin-7-glucoside and Chlorgenic acid were the most common constituents in the extract. RT-PCR results declared a significant up-regulation in mRNA gene expression of and upon treatment. Meanwhile, gene expression recorded a slight down-regulation. Gene expression of apoptotic biomarker demonstrated a significant down regulation as well as inflammatory biomarkers and .
From the data recorded, it could be concluded that exerts a great hypoglycemic potential via modulating , , and gene expression and signaling pathways and could be considered as an effective candidate for DMT2 treatment.
2型糖尿病（DMT2）是环境因素与某些遗传因素之间双重相互作用的结果。这凸显了对新型治疗策略的迫切需求。然而，（作为一种陆生草药被认为是天然抗氧化剂的重要来源，它可被评估为一种抗高血糖药物。
在本研究中，高效液相色谱法鉴定了（的活性成分，包括总多酚和黄酮类化合物。通过单剂量链脲佐菌素（STZ）（35mg/kg体重）诱导雄性Wistar白化大鼠患2型糖尿病。糖尿病诱导一周后，大鼠口服（500mg/kg体重），持续一个月。进行分子分析以研究（对调节ATP结合盒转运蛋白A1（）和G1（）基因的效率，此外还研究凋亡生物标志物、糖原合酶激酶-3β（）和细胞癌基因-fos（）基因。此外，还评估了炎症生物标志物、核因子κB（）和肿瘤坏死因子-α（）的基因表达。
酒精提取物除了含有许多活性成分外，还含有作为没食子酸的多酚和作为槲皮素的黄酮类化合物。芹菜素-7-葡萄糖苷和绿原酸是提取物中最常见的成分。逆转录聚合酶链反应结果表明，（处理后，和的mRNA基因表达显著上调。同时，基因表达略有下调。凋亡生物标志物的基因表达以及炎症生物标志物和均显著下调。
根据记录的数据可以得出结论，（通过调节、、和的基因表达及信号通路发挥强大的降血糖潜力，可被视为DMT2治疗的有效候选药物。
