Department of Surgery, Nisantasi University, Istanbul, Turkey.
Department of Medical Biotechnology, Acibadem University, Istanbul, Turkey.
Korean J Intern Med. 2021 Mar;36(Suppl 1):S44-S52. doi: 10.3904/kjim.2019.050. Epub 2020 Jun 19.
BACKGROUND/AIMS: The aim of this study is to investigate the effects of the Hericium erinaceum on an experimental colitis model.
Twenty-four Wistar albino were included in this study. Rats were divided into three groups. Group 1 (n = 8) was sham group. Group 2 is the group of chemically induced by intrarectal administration of trinitrobenzene sulfonic acid (TNBS) resulting in colitis. Group 3 (n = 8) is the group that was treated 7 days before and 7 days after with H. erinaceum resulting in colitis. The activity of colitis was evaluated macroscopically and microscopically in rats. In other words, nitric oxide (NO) levels, malondialdehyde (MDA), interleukin 6 (IL-6), nuclear factor-kappa B (NF-κB) and, tumor necrosis factor-α (TNF-α) in addition to the myeloperoxidasem (MPO) activities was determined.
The rate of TNBS-induced colitis caused to increase the level of MDA activities meaningfully in the colitis group than the control group. The results indicated that MDA (p = 0.001), NO (p = 0.001), IL-6 (p = 0.001), MPO (p = 0.878), TNF-α (p = 0.001), and NF-κB levels of treatment group decreased in the blood and colon tissues because of the H. erinaceum treatment when compared to the colitis group. H. erinaceum treatment was related to the declining of MDA, NF-κB, NO, IL-6, and TNF-α levels.
H. erinaceum had a positive effect on the colitis by reducing oxidative damage in blood and tissue.
背景/目的：本研究旨在探讨猴头菇对实验性结肠炎模型的影响。
本研究纳入 24 只 Wistar 白化大鼠。将大鼠分为三组。第 1 组（n = 8）为假手术组。第 2 组为经直肠内给予三硝基苯磺酸（TNBS）诱导结肠炎的化学诱导组。第 3 组（n = 8）为在结肠炎发生前 7 天和后 7 天用猴头菇治疗的组。用宏观和微观方法评估大鼠结肠炎的活动度。换句话说，测定了一氧化氮（NO）水平、丙二醛（MDA）、白细胞介素 6（IL-6）、核因子-κB（NF-κB）和肿瘤坏死因子-α（TNF-α）以及髓过氧化物酶（MPO）活性。
TNBS 诱导的结肠炎导致结肠炎组 MDA 活性水平明显升高，与对照组相比。结果表明，与结肠炎组相比，治疗组血液和结肠组织中的 MDA（p = 0.001）、NO（p = 0.001）、IL-6（p = 0.001）、MPO（p = 0.878）、TNF-α（p = 0.001）和 NF-κB 水平因猴头菇治疗而降低。猴头菇治疗与 MDA、NF-κB、NO、IL-6 和 TNF-α水平的降低有关。
猴头菇通过减少血液和组织中的氧化损伤对结肠炎有积极作用。
